Tohyama K, Ueda T, Yoshida Y, Nakamura T
First Department of Internal Medicine, Fukui Medical School, Japan.
Exp Hematol. 1994 Jul;22(7):539-45.
To evaluate the effects of colony-stimulating factors (CSFs) on pathological cells from myelodysplastic syndromes (MDS), the blast cells from 19 MDS patients (eight low-risk MDS, six high-risk MDS, and five leukemic transformation of MDS [LT-MDS]) and four normal volunteers were successfully enriched by separating CD34-positive cells by the use of immunomagnetic beads, and their responsiveness to granulocyte or granulocyte-macrophage CSF (G-CSF or GM-CSF) was examined in short-term liquid suspension culture. The proliferation of MDS blast cells was clearly promoted by these CSFs in all cases examined, but considerable percentages of them often remained immature compared with the favorable maturation of normal blast cells, especially in the more advanced disease groups (LT-MDS and high-risk MDS) that included two prominent cases with a remarkable blast cell growth without maturation induction by CSFs. The expression of esterase activities was rather sluggish in the MDS cases, in contrast to normal expression. These data showed that MDS blast cells proliferate in response to CSFs but that maturation is less than that observed with normal blast cells in vitro. Much care should be taken with in vivo application of CSFs to high-risk MDS patients.
为评估集落刺激因子（CSF）对骨髓增生异常综合征（MDS）病理细胞的影响，通过使用免疫磁珠分离CD34阳性细胞，成功富集了19例MDS患者（8例低危MDS、6例高危MDS和5例MDS白血病转化[LT-MDS]）的原始细胞以及4名正常志愿者的原始细胞，并在短期液体悬浮培养中检测了它们对粒细胞集落刺激因子或粒细胞-巨噬细胞集落刺激因子（G-CSF或GM-CSF）的反应性。在所有检测病例中，这些CSF均明显促进了MDS原始细胞的增殖，但与正常原始细胞的良好成熟相比，相当比例的MDS原始细胞通常仍不成熟，尤其是在包括两例突出病例的更晚期疾病组（LT-MDS和高危MDS）中，这些病例的原始细胞生长显著但未被CSF诱导成熟。与正常表达相比，MDS病例中酯酶活性的表达相当迟缓。这些数据表明，MDS原始细胞对CSF有增殖反应，但在体外其成熟程度低于正常原始细胞。在对高危MDS患者进行CSF的体内应用时应格外谨慎。
