Silver R I, Wiley E L, Davis D L, Thigpen A E, Russell D W, McConnell J D
Department of Urology, University of Texas Southwestern Medical Center, Dallas.
J Urol. 1994 Aug;152(2 Pt 1):433-7. doi: 10.1016/s0022-5347(17)32757-x.
The androgen dihydrotestosterone is synthesized by the enzyme steroid 5 alpha-reductase, and it is required for growth and development of the prostate. We used immunohistochemistry to examine the expression of the type 2 isozyme of 5 alpha-reductase in benign prostatic hyperplasia and prostate cancer. The type 2 isozyme is highly expressed within stromal cells in both disease states. No type 2 isozyme is detectable in a lymph node metastasis. Immunoblotting studies show that androgen ablation therapies substantially decrease isozyme expression in the epididymis but have a lesser effect on expression in the prostate. Finasteride therapy (2 weeks to 3 years) did not abolish expression of the prostatic type 2 isozyme nor did this drug treatment induce expression of the type 1 isozyme.
雄激素双氢睾酮由甾体5α-还原酶合成，它是前列腺生长和发育所必需的。我们采用免疫组织化学方法检测5α-还原酶2型同工酶在良性前列腺增生和前列腺癌中的表达。在这两种疾病状态下，2型同工酶在基质细胞中均高表达。在淋巴结转移灶中未检测到2型同工酶。免疫印迹研究表明，雄激素去除疗法可显著降低附睾中同工酶的表达，但对前列腺中同工酶表达的影响较小。非那雄胺治疗（2周至3年）并未消除前列腺2型同工酶的表达，且该药物治疗也未诱导1型同工酶的表达。
