Harvey A N, Costa N D, Savage J R
MRC Radiobiology Unit, Chilton, Didcot, UK.
Mutat Res. 1994 Jul;315(1):17-23. doi: 10.1016/0921-8777(94)90023-x.
CHO (Chinese hamster ovary), xrs5 (X-ray sensitive Chinese hamster) and HF19 (untransformed human fibroblast) cells, were exposed to a lethal dose of the restriction enzyme Pvu II during electroporation or poration with the bacterial toxin streptolysin O. The uptake of the exclusion dye trypan blue was used as a measure of poration and compared with survival as measured by subsequent colony formation. It was assumed that any surviving cells had not been permeabilized and therefore did not receive any restriction enzyme. Electroporation alone proved to be more cytotoxic to the cells, whilst streptolysin O was more efficient at permeabilizing both hamster and human cells.
中国仓鼠卵巢细胞（CHO）、X射线敏感的中国仓鼠细胞（xrs5）和未转化的人成纤维细胞（HF19），在电穿孔过程中或用细菌毒素链球菌溶血素O进行打孔时，暴露于致死剂量的限制性内切酶Pvu II。摄取排斥染料台盼蓝用作打孔的指标，并与后续集落形成所测定的存活率进行比较。假定任何存活的细胞都未被通透化，因此未接受任何限制性内切酶。单独的电穿孔对细胞的细胞毒性更大，而链球菌溶血素O在使仓鼠和人细胞通透化方面更有效。
