Kita M, Tong L J, Tanaka K, Imanishi J
Département de Microbiologie, Université Médicale de la Préfecture de Kyoto, Japan.
C R Seances Soc Biol Fil. 1993;187(3):414-9.
The expression of the cytokine genes was studied under physiological conditions in normal adult mice using RT-PCR method capable of detecting low levels of mRNA. Total RNA was prepared from brain, spinal cord, lung, spleen, liver and kidney of 6 to 8 week-old specific pathogen-free BALB/c mice by acid guanidinium thiocyanate-phenol-chloroform (AGPC) method. cDNA was synthesized by M-MLV reverse transcriptase, and amplified using the specific oligonucleotide primers for IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, TNF-alpha, IFN-alpha, IFN-beta and IFN-gamma by PCR method. Although IL-1 mRNA was detected in all the organs, IL-3 mRNA was not detected in any organs tested. IL-2 or IL-4 mRNA and IL-5 mRNA were produced only in spleen and lung, respectively. However, IL-6, TNF-alpha and IFN mRNA were detected in some different organs. These results suggest that many kinds of cytokine mRNA were produced in vivo under physiological conditions in normal mice.
采用能够检测低水平mRNA的逆转录聚合酶链反应（RT-PCR）方法，在生理条件下对正常成年小鼠细胞因子基因的表达进行了研究。通过酸性异硫氰酸胍-苯酚-氯仿（AGPC）法，从6至8周龄无特定病原体的BALB/c小鼠的脑、脊髓、肺、脾、肝和肾中制备总RNA。用M-MLV逆转录酶合成cDNA，并通过聚合酶链反应（PCR）方法，使用针对白细胞介素-1（IL-1）、白细胞介素-2（IL-2）、白细胞介素-3（IL-3）、白细胞介素-4（IL-4）、白细胞介素-5（IL-5）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、干扰素-α（IFN-α）、干扰素-β（IFN-β）和干扰素-γ（IFN-γ）的特异性寡核苷酸引物进行扩增。尽管在所有器官中均检测到IL-1 mRNA，但在所检测的任何器官中均未检测到IL-3 mRNA。IL-2或IL-4 mRNA以及IL-5 mRNA分别仅在脾脏和肺中产生。然而，在一些不同器官中检测到了IL-6、TNF-α和干扰素mRNA。这些结果表明，在正常小鼠的生理条件下，体内产生了多种细胞因子mRNA。
