Louis J C, Burnham P, Varon S
Department of Biology, University of California at San Diego, La Jolla 92093.
J Neurobiol. 1994 Feb;25(2):209-17. doi: 10.1002/neu.480250211.
We examined the effects of changes caused by the blocking of protein and RNA synthesis on neurite outgrowth from neurons of the central nervous system (CNS) in primary culture. Exposure to cycloheximide and actinomycin-D led to dramatic increases in the length of neurites in cultures of neurons from various rat or chick CNS regions. Inhibitor-induced neurite outgrowth was observed (1) from dopaminergic neurons in mixed cultures of the rat substantia nigra or (2) in pure cultures of rat and chick neurons grown on a polyornithine/laminin substratum. These results suggest that neurite outgrowth from CNS neurons is kept restricted, at least in culture, by the continuous production of a labile neurite-inhibiting protein intrinsic to the neurons, which rapidly decays following inhibition of protein or RNA synthesis.
我们研究了蛋白质和RNA合成受阻所引起的变化对原代培养的中枢神经系统（CNS）神经元轴突生长的影响。用环己酰亚胺和放线菌素-D处理后，来自不同大鼠或鸡中枢神经系统区域的神经元培养物中的轴突长度显著增加。在大鼠黑质混合培养物中的多巴胺能神经元或在聚鸟氨酸/层粘连蛋白基质上生长的大鼠和鸡神经元的纯培养物中，均观察到抑制剂诱导的轴突生长。这些结果表明，中枢神经系统神经元的轴突生长至少在培养中受到神经元内不稳定的轴突抑制蛋白持续产生的限制，该蛋白在蛋白质或RNA合成受到抑制后会迅速降解。
