Brodish R J, Kuvshinoff B W, Fink A S, McFadden D W
Department of Surgery, University of Cincinnati Medical Center, Ohio.
Pancreas. 1994 May;9(3):297-303. doi: 10.1097/00006676-199405000-00003.
The influence of extrapancreatic nerves on the inhibition of meal- and secretogogue-induced pancreatic secretion by galanin was studied in conscious dogs. Chronic pancreatic fistulae were created in five mongrel dogs and a second group of five dogs also underwent complete pancreatic denervation. After recovery, galanin dose response (150-1,200 pmol/kg/h) revealed that 600 pmol/kg/h was the lowest dose of galanin to significantly inhibit pancreatic exocrine secretion. Pancreatic responses to a mixed meal, cholecystokinin (CCK) dose response (12.5-200 ng/kg/h), and secretin dose response (16-500 ng/kg/h) were determined. The experiments were then replicated with a continuous background infusion of galanin (600 pmol/kg/h). Galanin inhibited meal-, CCK-, and secretin-induced bicarbonate outputs in both the innervated and denervated pancreas. Galanin also inhibited meal- and CCK-induced protein responses in both groups. We conclude that extrapancreatic nerves do not mediate the inhibitory effects of galanin.
在清醒犬中研究了胰腺外神经对甘丙肽抑制进餐和促分泌素诱导的胰腺分泌的影响。在五只杂种犬中建立慢性胰瘘，另一组五只犬也进行了完全胰腺去神经支配。恢复后，甘丙肽剂量反应（150 - 1200 pmol/kg/h）显示，600 pmol/kg/h是显著抑制胰腺外分泌的最低甘丙肽剂量。测定了胰腺对混合餐、胆囊收缩素（CCK）剂量反应（12.5 - 200 ng/kg/h）和促胰液素剂量反应（16 - 500 ng/kg/h）的反应。然后在持续背景输注甘丙肽（600 pmol/kg/h）的情况下重复实验。甘丙肽抑制了神经支配和去神经支配胰腺中由进餐、CCK和促胰液素诱导的碳酸氢盐输出。甘丙肽也抑制了两组中由进餐和CCK诱导的蛋白质反应。我们得出结论，胰腺外神经不介导甘丙肽的抑制作用。
