Balk S P, Burke S, Polischuk J E, Frantz M E, Yang L, Porcelli S, Colgan S P, Blumberg R S
Hematology-Oncology Division, Beth Israel Hospital, Harvard Medical School, Boston, MA 02215.
Science. 1994 Jul 8;265(5169):259-62. doi: 10.1126/science.7517575.
A major histocompatibility complex class Ib protein, CD1d, is expressed by human intestinal epithelial cells (IECs) and is a ligand for CD8+ T cells. CD1d was found to be expressed on the surface of human IECs as a 37-kilodalton protein that was beta 2-microglobulin (beta 2M) independent with no N-linked carbohydrate. Transfection into a beta 2M- cell line confirmed that CD1d could be expressed at the cell surface in the absence of beta 2M. These data indicate that IECs use a specialized pathway for CD1d synthesis and that a beta 2M-independent class Ib protein may be the normal ligand for some intestinal T cells.
一种主要组织相容性复合体I类b蛋白，即CD1d，由人肠道上皮细胞（IECs）表达，是CD8 + T细胞的配体。发现CD1d以一种37千道尔顿的蛋白形式表达于人IECs表面，该蛋白不依赖β2微球蛋白（β2M）且无N - 连接碳水化合物。转染到β2M缺陷细胞系证实，在没有β2M的情况下CD1d仍可在细胞表面表达。这些数据表明IECs利用一种特殊途径进行CD1d合成，且一种不依赖β2M的I类b蛋白可能是某些肠道T细胞的正常配体。
