Rongish B J, Torry R J, Tucker D C, Tomanek R J
Department of Anatomy, University of Iowa, Iowa City 52242.
J Vasc Res. 1994 Jul-Aug;31(4):205-15. doi: 10.1159/000159045.
Coronary neovascularization was studied following grafting of avascular hearts from gestation day-12 (E-12) rat embryos to the anterior eye chambers of adult rats. Volume densities (Vv) of vessels, myocytes, and the extracellular matrix (ECM) after 3-7, 14, 21, and 35 days in oculo were compared to Vv in hearts developing in utero at E-15, E-18, and E-20. The myocardium in both models exhibited similar vessel Vv and capillary developmental stages: (1) clustering of endothelial cells and red blood cells; (2) endothelial cell migration, and (3) tube formation/maturation. The Vv of myocytes increased while that of the ECM remained constant over time. Cross-species grafting utilizing species-specific antibodies determined that the majority, but not all, of the 10-day graft vasculature was of graft origin. Therefore, both de novo growth (vasculogenesis) and sprouting (angiogenesis) were occurring in oculo. Tracer molecules infused into host rats reached the outermost graft vessels only after 10 days in oculo, suggesting a functional link with the host circulation after this time. Thus, we have shown that both models exhibit similar: (1) vascular Vv; (2) shifts in Vv of nonvascular components; (3) stages of neovascularization, and (4) mechanisms of neovascularization. In conclusion, coronary neovascularization occurring in oculo closely mimics normal coronary vessel development.
将妊娠第12天（E-12）大鼠胚胎的无血管心脏移植到成年大鼠眼前房后，对冠状动脉新生血管形成进行了研究。将眼内3至7天、14天、21天和35天后血管、心肌细胞和细胞外基质（ECM）的体积密度（Vv）与子宫内E-15、E-18和E-20发育心脏中的Vv进行比较。两种模型中的心肌均表现出相似的血管Vv和毛细血管发育阶段：（1）内皮细胞和红细胞聚集；（2）内皮细胞迁移，以及（3）管形成/成熟。随着时间的推移，心肌细胞的Vv增加，而ECM的Vv保持恒定。利用物种特异性抗体进行的跨物种移植确定，10天移植物血管系统的大部分（但不是全部）来自移植物。因此，眼内同时发生了从头生长（血管生成）和芽生（血管生成）。注入宿主大鼠的示踪分子在眼内10天后才到达最外层的移植物血管，表明此时与宿主循环存在功能联系。因此，我们已经表明两种模型表现出相似的：（1）血管Vv；（2）非血管成分Vv的变化；（3）新生血管形成阶段，以及（4）新生血管形成机制。总之，眼内发生的冠状动脉新生血管形成与正常冠状动脉发育非常相似。
