Jayaraman S, Muthukkaruppan V
Immunology. 1978 Feb;34(2):231-40.
Utilizing the capillary migration inhibition (MI) technique, the cell-mediated immune response to sheep erythrocytes (SRBC) has been studied in the lizard, . The viability of spleen cells in culture was above 85%, irrespective of the presence or absence of antigen. Both plaque-forming cell (PFC) and MI responses were found to be antigen-specific. Heat-labile serum factors did not seem to have a role in the MI phenomenon. Both cellular and sonicated membrane preparations of SRBC induced a similar pattern and degree of MI of sensitized spleen cells. Migration of spleen cells was observed within 1 h of the initiation of cultures. The maximum difference between control and experimental cultures occurred by 12 h of incubation. There was an insignificant escape from inhibition after 24 h of culture. Administration of 6×10 SRBC via the intramuscular route favoured both PFC and MI responses. Although the PFC generation was favoured, only a low level of MI was induced by the intraperitoneal and intracardiac injections. MI and PFC responses are inversely related to the amount of antigen injected. Administration of 10 SRBC resulted in a high degree of MI without the production of PFC. On the other hand, 6×10 SRBC produced an abundant PFC response with a lesser degree of MI. Incorporation of SRBC into Freund's complete adjuvant resulted in the production of MI response with a concurrent reduction in the number of PFC. Formalized SRBC generated a good MI response without the induction of PFC. Sonicated SRBC induced both PFC and MI responses. MI was shown to be mediated by sensitized lymphoid cells. As few as 5% sensitized spleen cells were enough to bring about significant MI of unsensitized spleen cells. Thus, MI has been shown to be an manifestation of CMI to SRBC in the lizard.
利用毛细血管迁移抑制（MI）技术，在蜥蜴中研究了对绵羊红细胞（SRBC）的细胞介导免疫反应。无论有无抗原，培养的脾细胞活力均高于85%。发现空斑形成细胞（PFC）反应和MI反应均具有抗原特异性。热不稳定血清因子似乎在MI现象中不起作用。SRBC的细胞制剂和超声破碎膜制剂诱导致敏脾细胞产生相似模式和程度的MI。在培养开始后1小时内观察到脾细胞的迁移。对照培养和实验培养之间的最大差异在孵育12小时时出现。培养24小时后抑制作用的逃逸不明显。经肌肉途径注射6×10个SRBC有利于PFC反应和MI反应。虽然PFC生成受到促进，但腹腔内和心内注射仅诱导低水平的MI。MI反应和PFC反应与注射的抗原量呈负相关。注射10个SRBC导致高度的MI反应而不产生PFC。另一方面，6×10个SRBC产生丰富的PFC反应，MI程度较低。将SRBC掺入弗氏完全佐剂中导致MI反应的产生，同时PFC数量减少。甲醛处理的SRBC产生良好的MI反应而不诱导PFC。超声破碎的SRBC诱导PFC反应和MI反应。MI反应显示由致敏淋巴细胞介导。低至5%的致敏脾细胞就足以引起未致敏脾细胞的显著MI反应。因此，MI反应已被证明是蜥蜴对SRBC的细胞介导免疫的一种表现。
