Cattini R, Robinson D, Gill O, Jolley N, Bacarese-Hamilton T
Serono Diagnostics Limited, Woking, Surrey, United Kingdom.
Eur J Clin Chem Clin Biochem. 1994 Mar;32(3):181-5.
This paper summarises the results of a comparison of the Serono SR1, Ciba Corning ACS 180, Abbott IMx and Hybritech Tandem-R prostate-specific antigen assays. One hundred serum pools were assayed using the four methods. Linear regression analysis of the data showed that, although overall correlations were good, different assays gave different prostate-specific antigen concentrations. Tandem-R and SR1 assays gave very similar prostate-specific antigen values; in general, the ACS assay gave higher prostate-specific antigen values than the IMx assay gave lower prostate-specific antigen values than the established Tandem-R assay. Following fractionation of serum from prostate cancer patients, all immunoassays detected several immunoreactive prostate-specific antigen forms. The major immunoreactive form (> 88% of immunoreactivity) had an apparent molecular size of M(r) approximately 100,000 and is likely to be a complex of prostate-specific antigen with alpha 1-antichymotrypsin; two minor forms had apparent molecular sizes of M(r) approximately 30,000 (probably free prostate-specific antigen) and 200,000 (probably prostate-specific antigen complexed to high molecular mass anti-proteases). From this study there is no evidence that polyclonal/monoclonal antibody immunoassays are to be preferred to monoclonal/monoclonal antibody immunoassays for the determination of free prostate-specific antigen in serum.
本文总结了对赛诺菲SR1、西巴-康宁ACS 180、雅培IMx和海布里奇Tandem-R前列腺特异性抗原检测方法进行比较的结果。使用这四种方法对100份血清混合样本进行了检测。对数据的线性回归分析表明，尽管总体相关性良好，但不同的检测方法得出的前列腺特异性抗原浓度不同。Tandem-R和SR1检测方法得出的前列腺特异性抗原值非常相似；一般来说，ACS检测方法得出的前列腺特异性抗原值高于IMx检测方法，而IMx检测方法得出的前列腺特异性抗原值低于既定的Tandem-R检测方法。对前列腺癌患者的血清进行分级分离后，所有免疫检测方法均检测到几种免疫反应性前列腺特异性抗原形式。主要的免疫反应性形式（>88%的免疫反应性）的表观分子大小约为M(r) 100,000，可能是前列腺特异性抗原与α1-抗糜蛋白酶的复合物；两种次要形式的表观分子大小分别约为M(r) 30,000（可能是游离前列腺特异性抗原）和200,000（可能是与高分子量抗蛋白酶复合的前列腺特异性抗原）。从这项研究中没有证据表明在测定血清中游离前列腺特异性抗原时，多克隆/单克隆抗体免疫检测方法比单克隆/单克隆抗体免疫检测方法更具优势。
