Marchini B, Dolcher M P, Sabbatini A, Klein G, Migliorini P
Clinical Immunology Unit, University of Pisa, Italy.
J Autoimmun. 1994 Apr;7(2):179-91. doi: 10.1006/jaut.1994.1014.
Epstein-Barr virus (EBV) infection is associated with production of autoantibodies. The N-terminal 35-58 sequence of EBNA I, one of the nuclear antigens encoded by EBV, is highly homologous to the C-terminal 95-119 region of the ribonucleoprotein SmD. Autoantibodies specific for SmD are present only in systemic lupus (SLE) sera and are therefore considered a serological marker of SLE. We measured antibodies to the EBNA I 35-58 sequence in EBV-related diseases and in autoimmune disorders. Antibodies to the EBNA I 35-58 peptide were present in 30% of normal sera, 12% Burkitt lymphoma, 22% infectious mononucleosis, 25% rheumatoid arthritis, 38% SLE and 33% Sjogren's syndrome. Antibodies to the SmD 95-119 peptide were detectable in 32% of SLE sera, 17% infectious mononucleosis and 12% Burkitt lymphoma. The specificity of anti-EBNA I 35-58 antibodies affinity-purified from nine sera was analysed by means of an inhibition assay. Only anti-EBNA I 35-58 antibodies affinity-purified from SLE sera have a similar affinity for the viral peptide and the SmD C-terminal one; they also bind the recombinant SmD in western blot. The results indicate that antibodies to EBNA I 35-58 are produced in normals, in EBV-related diseases and in autoimmune disorder, but only SLE sera contain anti-viral antibodies cross-reactive with an autoantigen.
爱泼斯坦-巴尔病毒（EBV）感染与自身抗体的产生有关。EBV编码的核抗原之一EBNA I的N端35 - 58序列与核糖核蛋白SmD的C端95 - 119区域高度同源。针对SmD的自身抗体仅存在于系统性红斑狼疮（SLE）血清中，因此被视为SLE的血清学标志物。我们检测了EBV相关疾病和自身免疫性疾病中针对EBNA I 35 - 58序列的抗体。30%的正常血清、12%的伯基特淋巴瘤、22%的传染性单核细胞增多症、25%的类风湿关节炎、38%的SLE和33%的干燥综合征中存在针对EBNA I 35 - 58肽的抗体。在32%的SLE血清、17%的传染性单核细胞增多症和12%的伯基特淋巴瘤中可检测到针对SmD 95 - 119肽的抗体。通过抑制试验分析了从9份血清中亲和纯化的抗EBNA I 35 - 58抗体的特异性。只有从SLE血清中亲和纯化的抗EBNA I 35 - 58抗体对病毒肽和SmD C端肽具有相似的亲和力；它们在蛋白质印迹中也能结合重组SmD。结果表明，正常人群、EBV相关疾病患者和自身免疫性疾病患者均可产生针对EBNA I 35 - 58的抗体，但只有SLE血清中含有与自身抗原交叉反应的抗病毒抗体。
