转化生长因子-β1对自发性高血压大鼠和Wistar-Kyoto大鼠血管平滑肌细胞中血小板源性生长因子受体结合及基因表达的影响
Effect of transforming growth factor-beta 1 on platelet-derived growth factor receptor binding and gene expression in vascular smooth muscle cells from SHR and WKY rats.
作者信息
Agrotis A, Saltis J, Bobik A
机构信息
Baker Medical Research Institute, Alfred Hospital, Prahran, Victoria, Australia.
出版信息
Clin Exp Pharmacol Physiol. 1994 Feb;21(2):145-8. doi: 10.1111/j.1440-1681.1994.tb02484.x.
Abstract
- This study examined the effects of transforming growth factor-beta 1 (TGF-beta 1) on platelet-derived growth factor-BB (PDGF-BB)-stimulated DNA synthesis, [125I]-PDGF-BB receptor binding and PDGF-beta receptor mRNA expression in vascular smooth muscle cells (VSMC) isolated from Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR). 2. TGF-beta 1 inhibited by 40% DNA synthesis stimulated by PDGF-BB in VSMC from WKY rats but potentiated by 20% growth factor-stimulated DNA synthesis in VSMC from the SHR. 3. Since the difference in effect of TGF-beta 1 could not be attributed to differential regulation of [125I]-PDGF-BB binding activity and PDGF-beta receptor mRNA expression, it is suggested that alterations in intracellular signalling pathways may account for the differential effects of TGF-beta 1 on PDGF-BB-stimulated DNA synthesis in VSMC from SHR and WKY rats.
摘要
- 本研究检测了转化生长因子-β1（TGF-β1）对从Wistar-Kyoto（WKY）大鼠和自发性高血压大鼠（SHR）分离的血管平滑肌细胞（VSMC）中血小板衍生生长因子-BB（PDGF-BB）刺激的DNA合成、[125I]-PDGF-BB受体结合及PDGF-β受体mRNA表达的影响。2. TGF-β1抑制WKY大鼠VSMC中由PDGF-BB刺激的40%的DNA合成，但使SHR大鼠VSMC中生长因子刺激的DNA合成增强20%。3. 由于TGF-β1作用的差异不能归因于[125I]-PDGF-BB结合活性和PDGF-β受体mRNA表达的差异调节，提示细胞内信号通路的改变可能是TGF-β1对SHR和WKY大鼠VSMC中PDGF-BB刺激的DNA合成产生不同作用的原因。
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