Yell J A, Burge S M, Dean D
Department of Dermatology, Churchill Hospital, Oxford, U.K.
Br J Dermatol. 1994 Jul;131(1):40-7. doi: 10.1111/j.1365-2133.1994.tb08455.x.
We have examined the action of cantharidin on the skin of patients with Darier's disease, and used immunohistological techniques to determine the distribution of desmosomal components, keratin intermediate filaments, and proteases in cantharidin-induced blisters. Cantharidin induced acantholysis, but the presence of acantholysis did not trigger the development of the characteristic warty, dyskeratotic papules in patients with Darier's disease. The distribution of desmosomal components, keratins and proteases within the acantholytic keratinocytes in the cantharidin-induced blisters was similar to that previously found in acantholytic cells within lesions of Darier's disease: peripheral staining for extracellular desmosomal components was reduced; some desmosomal components were detected diffusely in the acantholytic cells; basal cell keratin markers were expressed by some suprabasal acantholytic cells, and plasminogen was detected in association with acantholytic cells. Cleavage of desmosomes did not reveal the underlying abnormality in Darier's disease.
我们研究了斑蝥素对 Darier 病患者皮肤的作用，并采用免疫组织学技术来确定斑蝥素诱导水疱中桥粒成分、角蛋白中间丝和蛋白酶的分布。斑蝥素可诱导棘层松解，但棘层松解的存在并未引发 Darier 病患者典型的疣状、角化不良丘疹的发展。斑蝥素诱导水疱中棘层松解角质形成细胞内桥粒成分、角蛋白和蛋白酶的分布与先前在 Darier 病皮损内棘层松解细胞中发现的分布相似：细胞外桥粒成分的外周染色减少；一些桥粒成分在棘层松解细胞中呈弥漫性检测到；一些基底层上方的棘层松解细胞表达基底细胞角蛋白标志物，并且在棘层松解细胞中检测到纤溶酶原。桥粒的裂解并未揭示 Darier 病潜在的异常情况。
