Westwood N B, Chung R, Emmerson A J, Pearson T C
Division of Haematology, United Medical School, St. Thomas' Hospital, London.
Br J Haematol. 1994 Mar;86(3):468-74. doi: 10.1111/j.1365-2141.1994.tb04775.x.
Circulating haemopoietic progenitor cells from premature infants were assessed for their ability to respond to interleukin 3, granulocyte-macrophage colony stimulating factor and stem cell factor (SCF) in vitro. All three cytokines increased the number of colonies derived from burst forming units erythroid (BFU-E), colony forming units granulocyte-macrophage (CFU-GM) and multi-lineage progenitors (CFU-Mix) grown in the presence of erythropoietin (Epo). The size and haemoglobin content of BFU-E derived colonies also increased in the presence of the cytokines. Of those tested, SCF was found to be the most potent additive to Epo for the enhanced growth of BFU-E and CFU-Mix. In short-term liquid cultures without Epo, SCF alone induced globin synthesizing cells. Progenitors from premature infants were at least as responsive to all three cytokines as those from healthy adults. The use of SCF in combination with Epo in the prevention or treatment of anaemia in premature infants warrants further investigation.
对来自早产儿的循环造血祖细胞进行了体外评估，以检测它们对白细胞介素3、粒细胞巨噬细胞集落刺激因子和干细胞因子（SCF）的反应能力。在促红细胞生成素（Epo）存在的情况下，所有这三种细胞因子均增加了源自红系爆式集落形成单位（BFU-E）、粒细胞巨噬细胞集落形成单位（CFU-GM）和多系祖细胞（CFU-Mix）的集落数量。在细胞因子存在的情况下，BFU-E衍生集落的大小和血红蛋白含量也有所增加。在测试的细胞因子中，发现SCF是Epo增强BFU-E和CFU-Mix生长的最有效添加剂。在没有Epo的短期液体培养中，单独的SCF可诱导珠蛋白合成细胞。早产儿的祖细胞对所有三种细胞因子的反应至少与健康成年人的祖细胞一样。联合使用SCF和Epo预防或治疗早产儿贫血值得进一步研究。
