Shanina N A, Sveĭkata A P, Zavalishin I A, Gel'fand V I
Biull Eksp Biol Med. 1993 Feb;115(2):169-73.
We used non-direct immunofluorescence microscopy, immunoblotting and affinity chromatography on A-protein Superose to study antibodies to neural tissue antigens in sera from 11 patients with ALS and from 10 healthy donors. In all sera the majoric antigens had molecular masses of 150-200kD, 70kD and 50kD. No consistent differences were found between ALS patients and controls. Antibodies to 50kD and 70kD proteins from patients with ALS were found to be mostly IgM, whereas antibodies from control sera were mostly IgG. Antibodies to high molecular weight proteins (150-200kD) in ALS and controls belonged to both classes of immunoglobulins. Immunoblotting studies of neural tissue proteins after treatment blots with alkaline phosphatase showed considerable decrease of antibodies binding to neural tissue antigens in sera of ALS patients. The same results were obtained by immunofluorescence assay. The alkaline phosphatase experiments suggest that in ALS patients the sera antibodies are directed mainly against phosphoepitopes in protein antigenic determinants of the neural tissue. This results can lead to conclusion of a role for the altered phosphorylation of the neural proteins in the ALS pathogenesis.
我们使用非直接免疫荧光显微镜、免疫印迹法以及A蛋白琼脂糖凝胶亲和层析法，研究了11例肌萎缩侧索硬化症（ALS）患者和10名健康供者血清中针对神经组织抗原的抗体。在所有血清中，主要抗原的分子量分别为150 - 200kD、70kD和50kD。未发现ALS患者与对照组之间存在一致的差异。ALS患者血清中针对50kD和70kD蛋白的抗体大多为IgM，而对照血清中的抗体大多为IgG。ALS患者和对照组中针对高分子量蛋白（150 - 200kD）的抗体属于两类免疫球蛋白。用碱性磷酸酶处理印迹后的神经组织蛋白免疫印迹研究表明，ALS患者血清中与神经组织抗原结合的抗体显著减少。免疫荧光测定也得到了相同的结果。碱性磷酸酶实验表明，在ALS患者中，血清抗体主要针对神经组织蛋白抗原决定簇中的磷酸表位。这一结果可得出神经蛋白磷酸化改变在ALS发病机制中起作用的结论。
