Magoul R, Dubourg P, Kah O, Tramu G
Laboratoire de Neurocytochimie Fonctionnelle, URA CNRS 339, Talence, France.
Neurosci Lett. 1994 Mar 14;169(1-2):149-53. doi: 10.1016/0304-3940(94)90378-6.
Anatomical relationships between tachykinin-containing terminals and neurons of the medial preoptic area that innervate the arcuate nucleus were studied using silver staining of the retrograde tracer wheat germ agglutinin-apoperoxidase-gold (WGA-ApoHRP-gold) complex injected in the arcuate nucleus and pre-embedding immunocytochemistry for neurokinin A (NKA). At the histological level, retrogradely labeled cells not stained for NKA were seen to be surrounded by numerous NKA-immunopositive punctate profiles, in particular in the dorsal part of the medial preoptic area. At the ultrastructural level, retrogradely labeled cell bodies and dendritic profiles displayed highly electron-dense silver particle accumulations over the cytoplasm. The were seen in synaptic contact with one or several NKA-immunoreactive axon terminals containing small clear vesicles and dense-cored vesicles. Such synapses were either symmetrical or asymmetrical. The occurrence of synaptic contacts between tachykinin terminals and cells innervating the arcuate nucleus in the medial preoptic region provides a morphological support for a tachykinergic regulation of preoptic afferences to the arcuate nucleus. These results suggest that tachykinins are implicated in the indirect control of neuronal activity in the arcuate nucleus notably via the preoptic area. Consequently, tachykinins are potentially able to regulate indirectly numerous neuroendocrine events involving the tuberoinfundibular system.
运用逆行示踪剂小麦胚芽凝集素-过氧化物酶-金（WGA-ApoHRP-金）复合物注射至弓状核，并对神经激肽A（NKA）进行包埋前免疫细胞化学染色，研究了含速激肽终末与内侧视前区支配弓状核的神经元之间的解剖学关系。在组织学水平上，可见未被NKA染色的逆行标记细胞被大量NKA免疫阳性点状结构所包围，尤其是在内侧视前区的背侧部分。在超微结构水平上，逆行标记的细胞体和树突轮廓在细胞质上显示出高度电子致密的银颗粒聚集。可见它们与一个或几个含有小清亮囊泡和致密核心囊泡的NKA免疫反应性轴突终末形成突触联系。这种突触要么是对称的，要么是不对称的。速激肽终末与内侧视前区支配弓状核的细胞之间存在突触联系，为速激肽能对内侧视前区至弓状核的传入神经进行调节提供了形态学支持。这些结果表明，速激肽尤其通过视前区参与对弓状核神经元活动的间接控制。因此，速激肽可能有能力间接调节涉及结节漏斗系统的众多神经内分泌事件。
