Moody D M, Brown W R, Challa V R, Block S M
Department of Radiology, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina 27157.
Pediatr Res. 1994 Apr;35(4 Pt 1):424-30. doi: 10.1203/00006450-199404000-00008.
We evaluated the utility of alkaline phosphatase (AP) histochemical staining for studying intraparenchymal vascular morphology in the brain of a 31-wk-gestation (1480 g) neonate who died of respiratory insufficiency after 23 h. In this baby, afferent cerebral vessels (arteries, arterioles, and capillaries) stained with AP, whereas efferent vessels (venules, veins) did not. The large periventricular channels in the germinal matrix were determined to be veins, according to AP staining criteria. Arterioles connected with these large periventricular veins after passing through 4- to 6-microns capillaries. Branchings and connections of the cerebral circulation were conventional; i.e. no arterial rete or arteriovenous shunts were found. With this method of differential vascular staining, bleeding in the germinal matrix was found to be perivenous only. No dilated capillaries or arterioles were seen. Smooth muscle was identified in extrastriatal medullary arteries. This preliminary investigation suggests that AP histochemical staining is an excellent method for studying brain vascular morphology and pathology of the very-low-birthweight neonate.
我们评估了碱性磷酸酶（AP）组织化学染色在研究一名孕31周（1480克）新生儿脑实质内血管形态方面的效用，该新生儿在出生23小时后死于呼吸功能不全。在这个婴儿中，传入性脑血管（动脉、小动脉和毛细血管）被AP染色，而传出性血管（小静脉、静脉）未被染色。根据AP染色标准，生发基质中的大室周通道被确定为静脉。小动脉在穿过4至6微米的毛细血管后与这些大室周静脉相连。脑循环的分支和连接是常规的，即未发现动脉网或动静脉分流。通过这种差异血管染色方法，发现生发基质中的出血仅为静脉周围出血。未见到扩张的毛细血管或小动脉。在纹状体外侧髓质动脉中鉴定出平滑肌。这项初步研究表明，AP组织化学染色是研究极低出生体重儿脑血管形态和病理的一种极好方法。
