Ranganathan S, Ganguly A K, Datta K
Biochemistry Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India.
Mol Reprod Dev. 1994 May;38(1):69-76. doi: 10.1002/mrd.1080380112.
Hyaluronic acid, a major component of the extracellular matrix, plays an important role in the regulation of different cellular processes, e.g., locomotion, cell-cell interaction during morphogenesis, and differentiation. Distribution of hyaluronic acid with respect to the role of sperm hyaluronidase in sperm penetration and gamete interaction is well established. In order to elucidate this mechanism, in our current study we have identified and demonstrated, for the first time, the presence of a 68-kDa cell surface hyaluronic acid binding glycoprotein (HABP) in spermatozoa of different species (rat, mice, bull, and human) by immunoblot analysis and indirect immunofluorescence using the polyclonal antibodies raised against purified HABP. Furthermore, we were able to demonstrate a differential distribution of 68-kDa HA binding protein on the sperm head, midpiece, and tail of different species. To identify its role in sperm function, we observed its declining pattern during epididymal maturation and also the inhibition of sperm-oolemmal adherence by pretreatment of the sperms with anti-HABP antibodies. We have further observed its in vivo phosphorylation in motile spermatozoa. All our data clearly indicate that sperm hyaluronan binding protein may have a specific role in sperm maturation, motility, and fertilization processes.
透明质酸是细胞外基质的主要成分，在调节不同细胞过程中发挥重要作用，例如运动、形态发生过程中的细胞间相互作用以及分化。关于精子透明质酸酶在精子穿透和配子相互作用中的作用，透明质酸的分布已得到充分证实。为了阐明这一机制，在我们目前的研究中，我们首次通过免疫印迹分析和间接免疫荧光，使用针对纯化的透明质酸结合蛋白（HABP）产生的多克隆抗体，鉴定并证明了不同物种（大鼠、小鼠、公牛和人类）精子中存在一种68 kDa的细胞表面透明质酸结合糖蛋白（HABP）。此外，我们能够证明不同物种精子头部、中段和尾部68 kDa透明质酸结合蛋白的分布存在差异。为了确定其在精子功能中的作用，我们观察到其在附睾成熟过程中的下降模式，以及用抗HABP抗体预处理精子对精子-卵膜粘附的抑制作用。我们还进一步观察到其在活动精子中的体内磷酸化。我们所有的数据清楚地表明，精子透明质酸结合蛋白可能在精子成熟、运动和受精过程中具有特定作用。
