精子活力起始因子是太平洋鲱鱼卵绒毛膜的一种次要成分。

Sperm motility initiation factor is a minor component of the Pacific herring egg chorion.

作者信息

Griffin Frederick J, Vines Carol A, Pillai Murali C, Yanagimachi Ryuzo, Cherr Gary N

机构信息

University of California, Davis, Bodega Marine Laboratory, PO Box 247, Bodega Bay, USA.

Sonoma State University, Rohnert Park, California, USA.

出版信息

Dev Growth Differ. 1996 Apr;38(2):193-202. doi: 10.1046/j.1440-169X.1996.t01-1-00009.x.

DOI:10.1046/j.1440-169X.1996.t01-1-00009.x

PMID:37282292

Abstract

Polyclonal antibodies were generated to the 105 kDa herring sperm motility initiation factor (SMIF) and used to explore the role of SMIF in sperm-egg interaction. Using sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunoblotting with SMIF antibodies, it was demonstrated that SMIF is present as a minor (4-7% of total chorion protein) component of the chorion. The major polypeptides in the chorion migrated at 117 kDa and in a grouping between 48-54 kDa, with other minor bands above and below. The only detectable glycosylated component was the 105 kDa band, which was resolved at two isoelectric points (8.22 and 8.31) after isoelectric focusing gel electrophoresis. Using antibodies to SMIF, fertilization was blocked, sperm motility was inhibited in vitro in the presence of solubilized SMIF and SMIF binding sites on sperm were localized. Lastly, SMIF was localized to the region of the herring egg that encircles the micropyle.

摘要

制备了针对105 kDa鲱鱼精子运动起始因子（SMIF）的多克隆抗体，并用于探究SMIF在精卵相互作用中的作用。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳和用SMIF抗体进行免疫印迹分析，结果表明SMIF作为绒毛膜的次要成分（占绒毛膜总蛋白的4-7%）存在。绒毛膜中的主要多肽迁移至117 kDa以及在48-54 kDa之间成组出现，上下还有其他次要条带。唯一可检测到的糖基化成分是105 kDa条带，在等电聚焦凝胶电泳后在两个等电点（8.22和8.31）处分离。使用针对SMIF的抗体，受精被阻断，在存在溶解的SMIF的情况下精子运动在体外受到抑制，并且精子上的SMIF结合位点被定位。最后，SMIF定位于鲱鱼卵围绕卵孔的区域。