抗核糖体失活蛋白石竹素32和商陆毒蛋白单克隆抗体的制备与鉴定

Production and characterization of monoclonal antibodies against the ribosome inactivating proteins dianthin32 and momochin.

作者信息

Porro G, Bonardi M A, Giovanetti E, Lento P, Modena D

机构信息

ITALFARMACO S.p.A., Centro Ricerche, Milano, Italy.

出版信息

Hybridoma. 1994 Apr;13(2):99-105. doi: 10.1089/hyb.1994.13.99.

DOI:10.1089/hyb.1994.13.99

PMID:7519581

Abstract

Female BALB/c mice were immunized with either dianthin32 or momochin, type 1 ribosome-inactivating proteins (RIPs) derived from Dianthus charyophyllus and Momordica cochinchinensis, respectively. Five anti-dianthin32 and 6 anti-momochin secreting hybridomas were obtained by somatic fusion of lymphocytes with myeloma cell line NS0. The monoclonal antibodies (MAbs) produced were highly specific, as demonstrated by cross-reactivity assays performed with taxonomically related and unrelated type 1 RIPs, and recognized different epitopes of the antigen. The affinity constant of anti-RIPs MAbs ranged between 10(8) M-1 and 10(10) M-1.

摘要

雌性BALB/c小鼠分别用石竹素32或苦瓜素进行免疫，这两种物质分别是源自石竹和罗汉果的1型核糖体失活蛋白（RIPs）。通过淋巴细胞与骨髓瘤细胞系NS0的体细胞融合，获得了5株分泌抗石竹素32抗体的杂交瘤和6株分泌抗苦瓜素抗体的杂交瘤。所产生的单克隆抗体（MAbs）具有高度特异性，这通过与分类学上相关和不相关的1型RIPs进行交叉反应试验得到证明，并且识别抗原的不同表位。抗RIPs单克隆抗体的亲和常数在10(8) M-1至10(10) M-1之间。