Aebischer I, Stämpfli M R, Zürcher A, Miescher S, Urwyler A, Frey B, Luger T, White R R, Stadler B M
Institute of Clinical Immunology, University of Bern, Switzerland.
Eur J Immunol. 1994 Aug;24(8):1908-13. doi: 10.1002/eji.1830240829.
We determined the effect of adrenocorticotropin hormone (ACTH) on the regulation of IgE synthesis. Depending on the concentration, ACTH enhanced or inhibited IgE synthesis in a culture system where IgE synthesis was induced with interleukin-4 (IL-4) and anti-CD40 monoclonal antibody in peripheral blood mononuclear cells. Similar effects on IgE synthesis were observed by adding ACTH-related peptides, e.g. corticotropin-releasing factor (CRF), the inducer of ACTH, or alpha-melanocyte stimulating hormone (alpha-MSH), a cleavage product of ACTH. However, ACTH had no effect on IgG or IgM synthesis in this culture system. ACTH did not act directly on either B or T cells as there was no influence on IgE synthesis in a system using purified B cells alone or co-cultured with T cells. The effect of ACTH on IgE synthesis was mediated by accessory cells. This was shown by priming purified CD14-positive monocytes with ACTH and reconstitution experiments. Therefore, these findings suggest that ACTH and the related peptides CRF and alpha-MSH can influence the microenvironment modulating an IL-4 and anti-CD40 monoclonal antibody driven class switching to IgE via accessory cells.
我们确定了促肾上腺皮质激素（ACTH）对IgE合成调节的影响。在由白细胞介素-4（IL-4）和抗CD40单克隆抗体诱导外周血单核细胞合成IgE的培养系统中，ACTH根据浓度的不同增强或抑制IgE的合成。通过添加ACTH相关肽，如促肾上腺皮质激素释放因子（CRF，ACTH的诱导物）或α-黑素细胞刺激素（α-MSH，ACTH的裂解产物），观察到对IgE合成有类似影响。然而，在该培养系统中，ACTH对IgG或IgM的合成没有影响。ACTH不会直接作用于B细胞或T细胞，因为在仅使用纯化B细胞或与T细胞共培养的系统中，其对IgE合成没有影响。ACTH对IgE合成的影响是由辅助细胞介导的。这通过用ACTH预处理纯化的CD14阳性单核细胞和重建实验得以证明。因此，这些发现表明，ACTH以及相关肽CRF和α-MSH可通过辅助细胞影响调节由IL-4和抗CD40单克隆抗体驱动的向IgE类别转换的微环境。
