Moneo I, Puente S, Subirats M, Ruiz A, Lozano M, González-Muñoz M
Centro de Investigación Clínica, Instituto de Salud Carlos III, Madrid.
Allergol Immunopathol (Madr). 1994 Mar-Apr;22(2):46-51.
We studied 98 patients with different parasitosis, without clinical symptoms of mite sensitization, most of them coming from Guinea. Histamine release to Dermatophagoides pteronyssinus was performed using whole blood. Specific IgE to the same antigen was measured by EAST, as well as by an immunodot with the same antigen extract employed for the histamine release test. Finally, the EAST positive sera were studied by immunoblotting. The presence of specific IgE by EAST could be proved in 31 patients, but these antibodies were nor detected by dot, blot and histamine release. On the other hand, only two patients showed a positive histamine release test to D. pteronyssinus and in these two cases the EAST to mites was negative. There was no relation between total IgE levels and specific IgE to mites. The presence of mite-specific IgE showed a significative association to the parasite Trichuris trichiura (odds ratio 3.09). This fact suggest that the specific IgE values found in this population can reflect some cross-reaction between parasites and allergens. It is the author's opinion that the same study should be performed in european patients in order to test the relationship between mite-specific antibodies and the presence of parasites, specially of Trichuris trichiura.
我们研究了98例患有不同寄生虫病的患者，他们没有螨虫致敏的临床症状，其中大多数来自几内亚。采用全血检测对粉尘螨的组胺释放情况。通过酶联免疫吸附试验（EAST）以及使用与组胺释放试验相同的抗原提取物进行免疫斑点法检测针对同一抗原的特异性IgE。最后，通过免疫印迹法研究EAST阳性血清。通过EAST可在31例患者中证实特异性IgE的存在，但这些抗体在斑点法、印迹法和组胺释放试验中均未检测到。另一方面，只有两名患者对粉尘螨的组胺释放试验呈阳性，而在这两例中针对螨虫的EAST试验为阴性。总IgE水平与针对螨虫的特异性IgE之间没有关联。螨虫特异性IgE的存在与寄生虫鞭虫呈显著相关性（优势比3.09）。这一事实表明，在该人群中发现的特异性IgE值可能反映了寄生虫与变应原之间的某种交叉反应。作者认为，应该对欧洲患者进行同样的研究，以检验螨虫特异性抗体与寄生虫尤其是鞭虫的存在之间的关系。
