Sanchez M R, Ramirez J A, Larriva-Sahd J, Rodriguez M H, Mancilla R, Ortiz-Ortiz L
Departamento de Inmunologia, Universidad Nacional Autonoma de Mexico.
Am J Trop Med Hyg. 1994 Jul;51(1):60-7. doi: 10.4269/ajtmh.1994.51.60.
Monoclonal antibodies were produced against Plasmodium vivax obtained from patients living in southeastern Mexico, where P. vivax malaria is endemic. Nine hybridomas specific for this parasite were obtained. By an indirect immunofluorescence assay, seven antibodies were found to react with epitopes present in the cytoplasm of the infected erythrocyte and two with the parasite itself. By immunoblotting, five monoclonal antibodies reacted with a 17-kD protein band, three with an 85-kD band, and two with one of 45 kD. By immunogold electron microscopy, two antibodies that reacted with the cytoplasm of infected erythrocytes by immunofluorescence also labeled cytoplasmic clefts, and one, in addition, recognized caveola-vesicle complexes and the parasite matrix. These results demonstrate the value of monoclonal antibodies in identifying P. vivax antigens and disclosing their subcellular distribution.
针对从墨西哥东南部（间日疟原虫疟疾流行地区）患者体内获取的间日疟原虫制备了单克隆抗体。获得了9种针对该寄生虫的杂交瘤。通过间接免疫荧光测定法，发现7种抗体与感染红细胞胞质中存在的表位发生反应，2种与寄生虫本身发生反应。通过免疫印迹法，5种单克隆抗体与一条17-kD蛋白带发生反应，3种与一条85-kD带发生反应，2种与45 kD中的一条发生反应。通过免疫金电子显微镜观察，通过免疫荧光与感染红细胞胞质发生反应的2种抗体也标记了胞质裂隙，另外1种还识别小窝-囊泡复合体和寄生虫基质。这些结果证明了单克隆抗体在鉴定间日疟原虫抗原及其亚细胞分布方面的价值。
