Gross D R, Fiscus R R, Arden W A, Maley R H, Lanzo S, Salley R K
Department of Surgery, University of Kentucky College of Medicine, Lexington 40536-0084.
Neuropeptides. 1994 May;26(5):329-41. doi: 10.1016/0143-4179(94)90118-x.
Careful handling and preparation of freshly harvested vessels from 22 pigs and 12 rabbits revealed a two-phase vasorelaxation response to cumulative doses of substance P (SP). A rapid, transient relaxation was observed during the cumulative dose-response and a new plateau of equilibrium was seen following an increase in developed force after the last dose of SP. The phase 2 response is also produced by submaximal doses of SP and is not altered by pretreatment of the rings with Indomethacin. Acetylcholine (ACh) caused an endothelium-dependent relaxation but without evidence of a phase 2 plateau. N omega-Nitro-L-Arginine (L-NNA) and N omega-Nitro-L-Arginine Methylester (L-NAME) pretreatment resulted in a shift to the right in the phase 1 response to SP and a complete blockade of phase 2. Methylene blue caused nearly complete block of both phases. Nitroglycerin caused a dose-dependent and prolonged vasorelaxation with no phase 2.
对从22头猪和12只兔子身上刚获取的血管进行仔细处理和准备后发现，血管对累积剂量的P物质（SP）呈现出双相血管舒张反应。在累积剂量反应过程中观察到快速、短暂的舒张，在最后一剂SP后张力增加后出现了新的平衡平台期。第二阶段反应也可由亚最大剂量的SP产生，且用吲哚美辛预处理血管环后该反应不变。乙酰胆碱（ACh）引起内皮依赖性舒张，但无第二阶段平台期的迹象。用Nω-硝基-L-精氨酸（L-NNA）和Nω-硝基-L-精氨酸甲酯（L-NAME）预处理导致对SP的第一阶段反应向右偏移，并完全阻断第二阶段。亚甲蓝几乎完全阻断了两个阶段。硝酸甘油引起剂量依赖性和持续性血管舒张，无第二阶段。
