Maśliński C, Wyczółkowska J, Czuwaj M, Michoń T
Department of Biogenic Amines, Polish Academy of Sciences, Lódź.
Acta Pol Pharm. 1993;50(6):469-74.
The ability of Tołpa Peat Preparation (TPP) to induce or enhance an allergic sensitization was tested on mice and guinea pigs. The levels of IgE antibody in the mouse sera and IgG1 as well as IgE antibody levels in guinea pig sera were evaluated by PCA (Passive Cutaneous Anaphylaxis) tests. TPP adsorbed on aluminium hydroxide gel (alum) and introduced into BALB/c mice by several subcutaneous injections was unable to stimulate the noticeable anti-TPP IgE antibody response. TPP introduced together with ovalbumin (OA) into the mice in the course of immunization with OA did not enhance anti-OA IgE antibody response. TPP adsorbed on alum and injected subcutaneously into guinea pigs was unable to induce noticeable IgG1a, IgG1b and IgE antibody response, and mast cells obtained from lung and mesentery of these animals did not release histamine when challenged with TPP in vitro at 37 degrees C. In conclusion, our results show that under the experimental conditions used in the present experiments TPP was unable to induce or enhance an allergic sensitization of mice and guinea pigs.
在小鼠和豚鼠身上测试了托尔帕泥炭制剂（TPP）诱导或增强过敏致敏的能力。通过被动皮肤过敏反应（PCA）试验评估小鼠血清中IgE抗体水平以及豚鼠血清中IgG1和IgE抗体水平。吸附在氢氧化铝凝胶（明矾）上并通过多次皮下注射引入BALB/c小鼠体内的TPP，无法刺激明显的抗TPP IgE抗体反应。在以卵清蛋白（OA）免疫小鼠的过程中，将TPP与OA一起引入小鼠体内，并未增强抗OA IgE抗体反应。吸附在明矾上并皮下注射到豚鼠体内的TPP，无法诱导明显的IgG1a、IgG1b和IgE抗体反应，并且从这些动物的肺和肠系膜中获得的肥大细胞在37℃体外受到TPP刺激时不会释放组胺。总之，我们的结果表明，在本实验所采用的实验条件下，TPP无法诱导或增强小鼠和豚鼠的过敏致敏。
