Elstad C A, Meadows G G, Aslakson C J, Starkey J R
Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, Pullman 99164.
Adv Exp Med Biol. 1994;354:171-83. doi: 10.1007/978-1-4899-0939-8_13.
We have shown that Tyr and Phe restriction suppresses the malignant phenotype of the highly invasive and metastatic BL6 variant of B16 murine melanoma. Lung-colonizing abilities of Tyr- and Phe-modulated in vivo and in vitro variants of BL6 are inhibited following intravenous inoculation into mice fed normal diet. Although this antimetastatic effect of Tyr and Phe restriction is most likely not due to differences in attachment to endothelium, our data indicate that major impacts of Tyr and Phe restriction are at the level of the tumor, itself. Modulation of host immune responses, which in turn suppresses metastasis, does not appear to contribute significantly to the altered phenotype. Although numbers and function of T cells, mast cells, and NK cells are affected by Tyr and Phe restriction, they are not involved in the Tyr- and Phe-mediated suppression of tumor growth, metastasis, or angiogenesis. Our data do not rule out the importance of other host factors involved in the Tyr and Phe modulation of tumor phenotype. The outcome of this modulation results most likely from complex Tyr/Phe-tumor-host interactions.
我们已经表明，酪氨酸（Tyr）和苯丙氨酸（Phe）限制可抑制B16小鼠黑色素瘤高侵袭性和转移性BL6变体的恶性表型。在给喂食正常饮食的小鼠静脉接种后，Tyr和Phe调节的BL6体内外变体的肺定植能力受到抑制。虽然Tyr和Phe限制的这种抗转移作用很可能不是由于与内皮细胞附着的差异，但我们的数据表明，Tyr和Phe限制的主要影响在于肿瘤本身水平。宿主免疫反应的调节反过来抑制转移，但似乎对改变的表型没有显著贡献。虽然T细胞、肥大细胞和自然杀伤（NK）细胞的数量和功能受到Tyr和Phe限制的影响，但它们不参与Tyr和Phe介导的肿瘤生长、转移或血管生成的抑制。我们的数据不排除其他宿主因素在Tyr和Phe调节肿瘤表型中的重要性。这种调节的结果很可能源于复杂的Tyr/Phe-肿瘤-宿主相互作用。
