各种药物（星形孢菌素、除莠霉素A、酮替芬、茶碱、FK506和环孢菌素A）对嗜酸性粒细胞活力的影响。

Effects of various drugs (staurosporine, herbimycin A, ketotifen, theophylline, FK506 and cyclosporin A) on eosinophil viability.

作者信息

Hossain M, Okubo Y, Sekiguchi M

机构信息

First Department of Internal Medicine, Shinshu University School of Medicine.

出版信息

Arerugi. 1994 Jun;43(6):711-7.

PMID:7520689

Abstract

Eosinophils are known to play an important role in the pathogenesis of asthma and other allergic diseases. This study demonstrated the effects of various drugs on eosinophil viability in vitro, which might help clinicians and researchers in treating and studying eosinophilic diseases. Staurosporine, a protein kinase C inhibitor, and herbimycin A, a tyrosine kinase inhibitor, at 10(-6) M and 10(-7) M significantly lowered eosinophil viability in a dose-dependent fashion (p < 0.002, p < 0.02 and p < 0.001, p < 0.002, respectively). Both staurosporine and herbimycin A reduced eosinophil survival in a time-dependent fashion at 10(-6) M and 10(-7) M. Ketotifen at 10(-4) M and theophylline at 10(-3) M, significantly decreased eosinophil viability (p < 0.001 and p < 0.001, respectively) in the presence of 100 pg/ml of recombinant human interleukin-5 (rhIL-5). Both FK506 and cyclosporin A at 10(-4) M significantly reduced eosinophil viability (p < 0.001 and p < 0.005, respectively) in the presence of 100 pg/ml of rhIL-5. Our data show that ketotifen, theophylline, FK506, cyclosporin A reduced eosinophil viability at a high concentration. Furthermore, it is suggested that protein kinase C and tyrosine kinase are involved in eosinophil survival.

摘要

众所周知，嗜酸性粒细胞在哮喘和其他过敏性疾病的发病机制中发挥着重要作用。本研究展示了各种药物在体外对嗜酸性粒细胞活力的影响，这可能有助于临床医生和研究人员治疗和研究嗜酸性粒细胞疾病。蛋白激酶C抑制剂星形孢菌素和酪氨酸激酶抑制剂赫曲霉素A，在浓度为10⁻⁶ M和10⁻⁷ M时，以剂量依赖性方式显著降低嗜酸性粒细胞活力（分别为p < 0.002，p < 0.02和p < 0.001，p < 0.002）。星形孢菌素和赫曲霉素A在10⁻⁶ M和10⁻⁷ M时均以时间依赖性方式降低嗜酸性粒细胞存活率。在存在100 pg/ml重组人白细胞介素-5（rhIL-5）的情况下，10⁻⁴ M的酮替芬和10⁻³ M的茶碱显著降低嗜酸性粒细胞活力（分别为p < 0.001和p < 0.001）。在存在100 pg/ml rhIL-5的情况下，10⁻⁴ M的FK506和环孢素A均显著降低嗜酸性粒细胞活力（分别为p < 0.001和p < 0.005）。我们的数据表明，酮替芬、茶碱、FK506、环孢素A在高浓度下会降低嗜酸性粒细胞活力。此外，提示蛋白激酶C和酪氨酸激酶参与嗜酸性粒细胞的存活。