Burdon T G, Demmer J, Clark A J, Watson C J
Division of Molecular Biology, Roslin Institute (Edinburgh), Midlothian, UK.
FEBS Lett. 1994 Aug 22;350(2-3):177-82. doi: 10.1016/0014-5793(94)00757-8.
Site-directed mutagenesis of the three binding sites for the mammary factor MPBF in the beta-lactoglobulin (BLG) promoter demonstrates that MPBF is a transcriptional activator of the BLG gene in mammary cells. MPBF requires phosphorylation on tyrosine for maximum binding activity and binds to GAS (interferon gamma-activation site) elements which are similar to the MPBF binding sites. Prolactin induces MPBF binding activity in CHO cells and is not antigenically related to Stat1 (p91) and Stat2 (p113), suggesting that this transcription factor is likely to be another member of the STAT family of cytokine/growth factor-induced transcription factors.
对β-乳球蛋白（BLG）启动子中乳腺因子MPBF的三个结合位点进行定点诱变，结果表明MPBF是乳腺细胞中BLG基因的转录激活因子。MPBF需要酪氨酸磷酸化才能达到最大结合活性，并与类似于MPBF结合位点的GAS（γ-干扰素激活位点）元件结合。催乳素可诱导CHO细胞中的MPBF结合活性，且与Stat1（p91）和Stat2（p113）无抗原相关性，这表明该转录因子可能是细胞因子/生长因子诱导的转录因子STAT家族的另一个成员。
