Durante W, Cheng K, Schafer A I
Houston VA Medical Center, TX 77030.
Thromb Res. 1994 Jul 1;75(1):63-71. doi: 10.1016/0049-3848(94)90140-6.
Experiments were performed to examine the effect of cyclic nucleotides on the expression of inducible nitric oxide synthase (iNOS) activity by interleukin-1 beta (IL-1 beta) treated rat aortic smooth muscle cells (SMC). Treatment of vascular SMC with IL-1 beta stimulated iNOS mRNA expression and the subsequent release of nitrite, a stable oxidation product of nitric oxide (NO). Similarly, lipophilic analogues of cAMP also induced both iNOS mRNA expression and nitrite release. The addition of IL-1 beta and cAMP derivatives resulted in a synergistic enhancement of both iNOS mRNA production and of nitrite formation. In contrast, lipophilic analogues of cGMP did not induce iNOS expression. The addition of cGMP derivatives modestly increased IL-1 beta-induced SMC nitrite generation without affecting the production of iNOS mRNA. The capacity of cyclic nucleotides to positively modulate the induction of iNOS activity may play an important role in regulating the release of NO in vivo.
进行实验以研究环核苷酸对经白细胞介素 -1β（IL -1β）处理的大鼠主动脉平滑肌细胞（SMC）中诱导型一氧化氮合酶（iNOS）活性表达的影响。用IL -1β处理血管平滑肌细胞可刺激iNOS mRNA表达以及随后亚硝酸盐的释放，亚硝酸盐是一氧化氮（NO）的一种稳定氧化产物。同样，cAMP的亲脂性类似物也诱导iNOS mRNA表达和亚硝酸盐释放。添加IL -1β和cAMP衍生物导致iNOS mRNA产生和亚硝酸盐形成的协同增强。相比之下，cGMP的亲脂性类似物不诱导iNOS表达。添加cGMP衍生物适度增加了IL -1β诱导的SMC亚硝酸盐生成，但不影响iNOS mRNA的产生。环核苷酸正向调节iNOS活性诱导的能力可能在体内调节NO释放中起重要作用。
