Hirobe T
Division of Biology, National Institute of Radiological Sciences, Chiba, Japan.
Mutat Res. 1994 Sep;322(3):213-20. doi: 10.1016/0165-1218(94)90009-4.
Pregnant females C57BL/10JHir-p/p mice crossed with C57BL/10JHir males were whole-body irradiated with a single acute dose of 60Co-gamma-rays to investigate the effect of gamma-radiation on embryonic melanoblasts. The effect was studied by scoring changes in the cutaneous coats of F1 offspring 25 days after birth. White spots were found in mid-ventrum of the animals. Melanoblasts and melanocytes were not observed in the spotted skin. The frequency of the spots increased in a dose-dependent manner. White spots were found in mid-ventrum of (C57BL/6J female x C3H/HeJmsHir male) F1 exposed to gamma-rays. However, the frequency of the spots in (C57BL/6J female x C3H/HeJmsHir male) F1 were extremely lower than that in (C57BL/10JHir-p/p female x C57BL/10JHir male) F1, suggesting the possibility that the frequency of mid-ventral white spots are genetically controlled. Moreover, the highest frequency was found in (C57BL/10JHir-p/p female x C57BL/10JHir male) F1 irradiated at 8.5 days of gestation. This stage corresponds to the stage of initiation of neural-crest cell migration. These results indicate that gamma-radiation affects the differentiation of melanocytes in the skin both with genetical control and with greater effects seen at the stage of initiation of neural-crest cell migration.
将C57BL/10JHir-p/p雌性小鼠与C57BL/10JHir雄性小鼠杂交，对怀孕的雌性小鼠进行单次急性剂量的60Co-γ射线全身照射，以研究γ辐射对胚胎黑素母细胞的影响。通过对出生后25天的F1代后代皮肤表皮变化进行评分来研究这种影响。在动物的腹部中部发现了白斑。在有斑点的皮肤中未观察到黑素母细胞和黑素细胞。斑点的频率呈剂量依赖性增加。在接受γ射线照射的（C57BL/6J雌性×C3H/HeJmsHir雄性）F1代的腹部中部发现了白斑。然而，（C57BL/6J雌性×C3H/HeJmsHir雄性）F1代中斑点的频率远低于（C57BL/10JHir-p/p雌性×C57BL/10JHir雄性）F1代，这表明腹部中部白斑的频率可能受遗传控制。此外，在妊娠8.5天时接受照射的（C57BL/10JHir-p/p雌性×C57BL/10JHir雄性）F1代中发现了最高频率。这个阶段对应于神经嵴细胞迁移开始的阶段。这些结果表明，γ辐射影响皮肤中黑素细胞的分化，既受遗传控制，且在神经嵴细胞迁移开始阶段影响更大。
