Srinivas U K, Revathi C J
Centre for Cellular and Molecular Biology, Hyderabad, India.
Biochem Mol Biol Int. 1994 May;33(1):21-8.
During rat liver development alpha-fetoprotein (AFP) and albumin (alb) are differentially regulated. Alpha-fetoprotein synthesis starts soon after fertilization whereas the earliest time albumin could be detected is day 17-18. However during normal development trace amounts of albumin mRNA could be detected on day 14 or 15. When 12-13 day old embryonic rat liver cells are given heat shock at 42 degrees C, there is an enhanced expression of albumin mRNA as well as protein. Under heat shock conditions, unlike albumin mRNA, there is specific degradation of alphafetoprotein mRNA. Western blot analysis indicated that AFP that was already present is not affected by heat shock whereas alpha-fetoprotein mRNA is degraded.
在大鼠肝脏发育过程中，甲胎蛋白（AFP）和白蛋白（alb）受到不同的调控。甲胎蛋白的合成在受精后不久就开始了，而最早能检测到白蛋白的时间是第17 - 18天。然而，在正常发育过程中，在第14或15天可以检测到微量的白蛋白mRNA。当12 - 13日龄的胚胎大鼠肝细胞在42℃下受到热休克时，白蛋白mRNA以及蛋白质的表达会增强。在热休克条件下，与白蛋白mRNA不同，甲胎蛋白mRNA会发生特异性降解。蛋白质印迹分析表明，已经存在的AFP不受热休克的影响，而甲胎蛋白mRNA会被降解。
