Matsui H, Tsuji S, Nishimura H, Nagasawa S
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
FEBS Lett. 1994 Sep 12;351(3):419-22. doi: 10.1016/0014-5793(94)00897-3.
Jurkat T cells die of apoptosis upon exposure to anti-Fas mAb. Here we show that although the alternative complement pathway generally does not attack homologous cells, anti-Fas-induced apoptotic Jurkat T cells were attacked antibody-independently by the alternative pathway of human complement and opsonized with iC3b, which is a ligand of the complement receptor type 3 (CR3) of phagocytes. These results suggest that apoptotic cells become the targets of the homologous alternative complement pathway, which facilitates the clearance of apoptotic cells by phagocytes.
Jurkat T细胞在暴露于抗Fas单克隆抗体后会因凋亡而死亡。我们在此表明，尽管替代补体途径通常不会攻击同源细胞，但抗Fas诱导凋亡的Jurkat T细胞会被人补体的替代途径独立于抗体进行攻击，并被iC3b调理，iC3b是吞噬细胞补体受体3（CR3）的配体。这些结果表明，凋亡细胞成为同源替代补体途径的靶标，这有助于吞噬细胞清除凋亡细胞。
