Deckert-Schlüter M, Schlüter D, Hof H, Wiestler O D, Lassmann H
Institut für Neuropathologie, Universitätskliniken Bonn, Germany.
J Neuropathol Exp Neurol. 1994 Sep;53(5):457-68. doi: 10.1097/00005072-199409000-00005.
Light microscopic and ultrastructural immunohistochemistry of cell adhesion molecules (CAMs) and major histocompatibility class II antigens (Ia) expression in experimental murine Toxoplasma encephalitis (TE) revealed a prominent upregulation of the intercellular cell adhesion molecule-1 (ICAM-1) and of Ia on cerebral endothelia, microglia, ependyma, and choroid plexus epithelium during acute and chronic TE. Microglia also expressed Mac-1 and leukocyte function-associated antigen-1 (LFA-1), which are both ligands of ICAM-1, as well as CD45. The prominent simultaneous expression of a multitude of these molecules on microglia is indicative of a central immunologic function of this cell type in TE. Additionally, occasional astrocytic processes slightly expressed Ia in full-blown TE. The vascular cell adhesion molecule-1 (VCAM-1) was restricted to endothelia of cerebral blood vessels, which frequently showed perivascular cuffing of inflammatory cells, ependyma, and choroid plexus epithelium. Upregulation of Ia, CAMs and their ligands correlated with disease activity. Immunohistochemical analysis of the functional state of infiltrating T cells showed a preferential recruitment of CD44+ memory and activated interleukin-2R+ T cells in TE.
对实验性鼠类弓形虫脑炎（TE）中细胞黏附分子（CAMs）和主要组织相容性复合体II类抗原（Ia）表达进行光镜和超微结构免疫组化分析发现，在急性和慢性TE期间，细胞间黏附分子-1（ICAM-1）以及Ia在脑内皮细胞、小胶质细胞、室管膜和脉络丛上皮细胞中显著上调。小胶质细胞还表达Mac-1和白细胞功能相关抗原-1（LFA-1），这两者均为ICAM-1的配体，同时也表达CD45。这些分子在小胶质细胞上大量同时表达，表明该细胞类型在TE中具有核心免疫功能。此外，在完全发展的TE中，偶尔可见星形细胞突起轻度表达Ia。血管细胞黏附分子-1（VCAM-1）局限于脑血管内皮细胞，这些内皮细胞常出现炎症细胞、室管膜和脉络丛上皮细胞的血管周围套袖状浸润。Ia、CAMs及其配体的上调与疾病活动相关。对浸润性T细胞功能状态的免疫组化分析显示，在TE中优先募集CD44+记忆性和活化的白细胞介素-2R+ T细胞。
