Okamoto M, Mori S, Ichimura M, Endo H
School of Health Sciences, Okayama University, Japan.
Neurosci Lett. 1994 May 19;172(1-2):51-4. doi: 10.1016/0304-3940(94)90660-2.
Protective effects of chondroitin sulfate proteoglycans (CSPGs) from rat's brain against delayed cell death induced by excitatory amino acids were examined in cultured neurons of the rat. CSPGs reduced delayed neuronal death induced by 10 min exposure to glutamate at a concentration between 100 microM and 1 mM when lactate dehydrogenase activity of culture medium was assayed 24 h after the exposure. CSPGs also protected neuronal death induced by 200 microM N-methyl-D-aspartate (NMDA), kainate or 100 microM alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA). CSPGs reduced death of cortical and hippocampal neurons even when they were administered at 2 h, but not 6 and 12 h, after the exposure to glutamate. These results indicate that CSPGs may have a neuroprotective action against acute noxious conditions in the brain.
在大鼠培养神经元中研究了大鼠脑硫酸软骨素蛋白聚糖（CSPGs）对兴奋性氨基酸诱导的延迟性细胞死亡的保护作用。当在暴露后24小时测定培养基的乳酸脱氢酶活性时，CSPGs减少了在100微摩尔至1毫摩尔浓度下暴露于谷氨酸10分钟所诱导的延迟性神经元死亡。CSPGs还保护由200微摩尔N-甲基-D-天冬氨酸（NMDA）、海人藻酸或100微摩尔α-氨基-3-羟基-5-甲基异恶唑-4-丙酸（AMPA）诱导的神经元死亡。即使在暴露于谷氨酸后2小时给予CSPGs，其也能减少皮质和海马神经元的死亡，但在6小时和12小时给予则不能。这些结果表明，CSPGs可能对脑中的急性有害状况具有神经保护作用。
