Leader D J, Connelly S, Filipowicz W, Brown J W
Scottish Crop Research Institute, Dundee, UK.
Biochim Biophys Acta. 1994 Sep 13;1219(1):145-7. doi: 10.1016/0167-4781(94)90257-7.
We have used a probe encoding a U3snRNA gene of Arabidopsis to isolate maize U3snRNA genomic sequences. Of two clones sequenced, one encodes a single U3 gene which has been shown to be expressed in transfected maize protoplasts. The second clone encodes a U3 related sequence which appears to be an RNA-mediated pseudogene.
我们使用了一个编码拟南芥U3snRNA基因的探针来分离玉米U3snRNA基因组序列。在对两个克隆进行测序后,其中一个克隆编码单个U3基因,该基因已被证明在转染的玉米原生质体中表达。第二个克隆编码一个与U3相关的序列,该序列似乎是一个RNA介导的假基因。