Donahue J P, Sugg N, Hawiger J
Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN 37232.
Biochim Biophys Acta. 1994 Sep 13;1219(1):228-32. doi: 10.1016/0167-4781(94)90278-x.
We isolated a 15.5 kilobase pair DNA fragment that contains the 5' end of the human vitronectin receptor alpha subunit (alpha v) gene. The nucleotide sequence of the 5' flanking region, first exon and part of the first intron of the alpha v gene was determined. The sequence showed that the 5' end of the alpha v gene lies within a CpG island. The transcriptional initiation site was mapped 169 base pairs upstream of the alpha v translational initiation site. The 5' flanking region of the alpha v gene does not contain TATA or InR transcriptional control elements but does contain four Sp1 binding sites, two Ets binding sites and one GATA binding site. The identified alpha v gene 5' flanking region directed the expression of human growth hormone in transfected HeLa cells. Successive deletions of the 5' flanking region demonstrated a 222 bp region that exerts a strong positive effect on alpha v promoter activity.
我们分离出一个15.5千碱基对的DNA片段，它包含人玻连蛋白受体α亚基（αv）基因的5′端。测定了αv基因5′侧翼区、第一个外显子和第一个内含子部分的核苷酸序列。该序列表明αv基因的5′端位于一个CpG岛内。转录起始位点定位在αv翻译起始位点上游169个碱基对处。αv基因的5′侧翼区不包含TATA或InR转录控制元件，但含有四个Sp1结合位点、两个Ets结合位点和一个GATA结合位点。鉴定出的αv基因5′侧翼区在转染的HeLa细胞中指导人生长激素的表达。对5′侧翼区进行连续缺失显示出一个对αv启动子活性有强烈正向作用的222 bp区域。
