Robert B, Antoine H, Dreze F, Coppe P, Collard A
Département de Virologie, Centre d' Economie Rurale, Marloie, Belgium.
Vet Res. 1994;25(4):384-98.
Crossreacting antigens between life cycle stages of Cryptosporidium parvum (Protozoa, Apicomplexa) were detected using monoclonal antibodies (mAbs). Shared epitopes were demonstrated by immunoelectron microscopy, at the level of micronemes of the sporozoite and merozoite stages; some dense granules were also labelled but not so intensively. The parasitophorous vacuole membranes of all intracellular stages, the wall-forming bodies of macrogametes and the outer oocyst walls all shared these epitopes. The antigens that bear these epitopes were characterized using the whole oocyst and sporozoite stages as sources of antigenic material. Complex labelling patterns were observed on Western blots. However, all the mAbs used in this study recognized an antigen of more than 500 kDa. The glycoproteinic nature of this antigen was demonstrated by its sensitivity to pronase and periodate treatments. The expression of this high molecular weight immunoreactive antigen in the intracellular stages of C parvum was not investigated and remains to be found.
利用单克隆抗体（mAb）检测微小隐孢子虫（原生动物，顶复门）生命周期各阶段之间的交叉反应抗原。通过免疫电子显微镜在子孢子和裂殖子阶段的微线体水平证实了共有表位；一些致密颗粒也被标记，但强度没那么高。所有细胞内阶段的寄生泡膜、大配子的壁状体和卵囊外壁都共有这些表位。以整个卵囊和子孢子阶段作为抗原物质来源，对带有这些表位的抗原进行了表征。在蛋白质印迹法上观察到复杂的标记模式。然而，本研究中使用的所有单克隆抗体都识别一种分子量超过500 kDa的抗原。该抗原对链霉蛋白酶和高碘酸盐处理敏感，证明了其糖蛋白性质。本研究未调查该高分子量免疫反应性抗原在微小隐孢子虫细胞内阶段的表达情况，仍有待发现。
