Rosenfeld C S
Western Pennsylvania Cancer Institute, West Penn Hospital, Pittsburgh 15224.
J Hematother. 1993 Winter;2(4):525-8. doi: 10.1089/scd.1.1993.2.525.
Standardization of protocols for processing peripheral blood stem cells (PBSC) will be important for multiinstitutional studies. For this reason, a strategy was developed utilizing the small volume collection chamber (SVCC) in the Fenwal CS-3000 PLUS Blood Cell Separator and cryopreservation without a rate controller in medium containing pentastarch. Use of the SVCC allows for a small extracorporeal volume, extraction of a minimal number of platelets, and a high efficiency of mononuclear cell (MNC) removal. Advantages of the pentastarch cryopreservation technique include reduction in technician time compared to controlled rate cryopreservation and use of lower concentrations in DMSO. Clinical studies using the SVCC and pentastarch technique demonstrate that these goals have been accomplished. Furthermore, patients transplanted with cells that were processed by this technique engrafted faster and were discharged sooner than patients receiving autologous bone marrow transplants.
对外周血干细胞（PBSC）处理方案进行标准化对于多机构研究而言至关重要。因此，我们制定了一项策略，即利用芬华CS - 3000 PLUS血细胞分离机中的小容量采集室（SVCC），并在含有喷他淀粉的培养基中进行无速率控制器的冷冻保存。使用SVCC可实现较小的体外循环血量、最少数量血小板的提取以及单核细胞（MNC）的高效去除。喷他淀粉冷冻保存技术的优势包括与程序控制冷冻保存相比减少了技术人员的操作时间，以及使用较低浓度的二甲基亚砜（DMSO）。采用SVCC和喷他淀粉技术的临床研究表明，这些目标均已实现。此外，接受以此技术处理的细胞进行移植的患者，其植入速度更快，出院时间也比接受自体骨髓移植的患者更早。
