Hall S E, Savill J S, Henson P M, Haslett C
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, United Kingdom.
J Immunol. 1994 Oct 1;153(7):3218-27.
The fate of neutrophils (PMNs) at sites of inflammation is important to our understanding of many disease processes. Previously, it had been widely assumed that extravasated PMNs inevitably disintegrated before their fragments were removed by local phagocytes, but we have recently described an alternative process whereby senescent PMNs undergo apoptosis (programmed cell death). This process leads to macrophage (Mphi) ingestion of the intact cell by a novel phagocytic recognition process. In this study, we show that monolayers of fibroblasts also can selectively phagocytose apoptotic PMNs and that the recognition of apoptotic PMNs by fibroblasts involves two distinct mechanisms: one uses the vitronectin receptor, as in Mphi ingestion of PMNs; the other uses a mannose/fucose-specific lectin, which plays no part in Mphi phagocytosis of apoptotic PMNs. The direct interactions between PMNs and fibroblasts demonstrated herein may have implications for our understanding of the relationship between inflammation and scarring in many diseases.
中性粒细胞（PMNs）在炎症部位的命运对于我们理解许多疾病过程至关重要。以前，人们普遍认为渗出的中性粒细胞在其碎片被局部吞噬细胞清除之前不可避免地会解体，但我们最近描述了另一种过程，即衰老的中性粒细胞会经历凋亡（程序性细胞死亡）。这个过程通过一种新的吞噬识别过程导致巨噬细胞（Mphi）摄取完整的细胞。在本研究中，我们表明成纤维细胞单层也可以选择性地吞噬凋亡的中性粒细胞，并且成纤维细胞对凋亡中性粒细胞的识别涉及两种不同的机制：一种使用玻连蛋白受体，如同巨噬细胞摄取中性粒细胞一样；另一种使用甘露糖/岩藻糖特异性凝集素，而它在巨噬细胞吞噬凋亡中性粒细胞的过程中不起作用。本文所展示的中性粒细胞与成纤维细胞之间的直接相互作用可能对我们理解许多疾病中炎症与瘢痕形成之间的关系具有启示意义。
