Lavabre-Bertrand T, Janossy G, Exbrayat C, Bourquard P, Duperray C, Navarro M
Department of Clinical Immunology, Royal Free Hospital School of Medicine, London, UK.
Leukemia. 1994 Sep;8(9):1557-63.
The CD5 antigen density on B cells was studied on fetal spleen, cord blood, and adult peripheral blood (after immunomagnetic bead purification) using an indirect immunofluorescence technique. In fetal spleen, there was a continuum in CD5 expression, whereas all cord blood and less than 20% adult peripheral blood B cells were CD5+. Mean CD5 antigen density on these normal cells was low (3-6 x 10(3) molecules/cell); eight to 20 times lower than on normal T lymphocytes. In adult blood, less than 10% B cells expressed more than 3 x 10(3) CD5 molecules/cell. In chronic malignancies, 34/35 cases had a CD5 antigen density lower than on residual T cells, but mean antigen density was higher (14.8 +/- 2.1 x 10(3) molecules/cell) than on normal B cells. Sixteen cases of chronic lymphocytic leukemia (50%) expressed a CD5 density above 10 x 10(3) molecules/cell. This aberrantly high CD5 expression was used to detect neoplastic cells after dilution in normal lymphocytes, with a limit of detection between 1:100 and 1:1000. Quantitation of the CD5 antigen allows better characterization of the B1 population and should be used for the monitoring of chronic malignancies.
采用间接免疫荧光技术，对胎儿脾脏、脐血和成人外周血（免疫磁珠纯化后）中的B细胞上的CD5抗原密度进行了研究。在胎儿脾脏中，CD5表达呈连续状态，而所有脐血和不到20%的成人外周血B细胞为CD5阳性。这些正常细胞上的平均CD5抗原密度较低（3 - 6×10³分子/细胞）；比正常T淋巴细胞低8至20倍。在成人血液中，不到10%的B细胞表达超过3×10³个CD5分子/细胞。在慢性恶性肿瘤中，35例中有34例的CD5抗原密度低于残留T细胞，但平均抗原密度（14.8±2.1×10³分子/细胞）高于正常B细胞。16例慢性淋巴细胞白血病（50%）表达的CD5密度高于10×10³分子/细胞。这种异常高的CD5表达被用于在正常淋巴细胞中稀释后检测肿瘤细胞，检测限在1:100至1:1000之间。CD5抗原的定量分析有助于更好地表征B1细胞群体，应用于慢性恶性肿瘤的监测。
