Polosa R, Crimi N, Prosperini G, Vancheri C, Magrì S, Mastruzzo C
Istituto di Malattie dell'Apparato Respiratorio, Università degli Studi di Catania.
Ann Ital Med Int. 1994 Apr-Jun;9(2):82-7.
Mediator release from activated mast cells is also likely to take place in the asthmatic airways in vivo during adenosine-induced bronchoconstriction. To test this hypothesis, we evaluated mast cell mediator release directly into the airways of 9 asthmatic subjects after endobronchial challenge with adenosine by bronchoalveolar lavage (BAL). The mediators measured were histamine, tryptase, and PGD2. When compared to the saline-challenged segment, the response to AMP instillation was characterized by a prompt reduction in airway calibre paralleled by a significant 4.2-fold increase in PGD2 levels in the BAL fluid (p = 0.004). There were also increases in median histamine (from 200.1 to 433.6 pg/mL) and tryptase levels (from 0.31 to 0.46 ng/mL) recovered after AMP challenge, although they were not significant. These findings support the view that acute bronchospastic response to AMP in asthmatic airways is paralleled by the local release of mast cells derived products, particularly PGD2.
在体内，腺苷诱导支气管收缩过程中，活化的肥大细胞释放介质也可能发生在哮喘气道中。为了验证这一假设，我们通过支气管肺泡灌洗（BAL），对9名哮喘患者进行支气管内腺苷激发试验后，直接评估肥大细胞介质释放到气道中的情况。所检测的介质为组胺、类胰蛋白酶和前列腺素D2（PGD2）。与盐水激发段相比，AMP滴注的反应特点是气道口径迅速减小，同时BAL液中PGD2水平显著增加4.2倍（p = 0.004）。AMP激发后回收的组胺中位数（从200.1 pg/mL增至433.6 pg/mL）和类胰蛋白酶水平（从0.31 ng/mL增至0.46 ng/mL）也有所增加，尽管不显著。这些发现支持这样一种观点，即哮喘气道对AMP的急性支气管痉挛反应与肥大细胞衍生产物特别是PGD2的局部释放同时发生。
