Yamamura Y
J Immunol. 1978 Jan;120(1):286-92.
The mode of production of specifically armed monocytic killer cells was investigated with the T1699 mammary adenocarcinoma in syngeneic DBA/2 mice. After overnight in vitro incubation of cells from the spleen but not from the lymph nodes, blood, or from the peritoneal cavity produced specific killer cells. The activation of spleen cells was inhibited by pretreatment with anti-theta serum and C; however, already activated specific killer cells were not sensitive to the same treatment. Removal of phagocytic cells did not significantly affect the cytotoxicity of the splenic killer cells whereas removal of rayon-wool adherent cells greatly reduced both the total cytotoxicity, and to a lesser extent, the cytotoxicity indices. Overnight co-cultivation of normal peritoneal-exudate cells with the lymph node cells from tumor-bearers, although neither class of cells alone was cytotoxic to T1699 cells in vitro, produced specific monocytic killer cells, through steps dependent on active T lymphocyte function. Culture spupernatants of tumor-bearer's spleen cells also contained factor(s) which induced cytotoxicity mediated by normal peritoneal-exudate cells against T1699 cells in vitro; and the production of the factor(s) was also inhibited by pretreatment of the spleen cells with anti-theta serum but not by anti-mouse IgG or anti-mouse whole gamma-globulins serum and C.
利用同基因DBA/2小鼠的T1699乳腺腺癌研究了特异性武装单核细胞杀伤细胞的产生方式。脾脏细胞而非淋巴结、血液或腹腔细胞经体外过夜培养后产生特异性杀伤细胞。抗θ血清和补体预处理可抑制脾细胞的激活；然而，已经激活的特异性杀伤细胞对相同处理不敏感。去除吞噬细胞对脾杀伤细胞的细胞毒性没有显著影响，而去除棉绒黏附细胞则大大降低了总细胞毒性，并在较小程度上降低了细胞毒性指数。正常腹腔渗出细胞与荷瘤动物淋巴结细胞过夜共培养，尽管单独一类细胞在体外对T1699细胞均无细胞毒性，但通过依赖活性T淋巴细胞功能的步骤产生了特异性单核细胞杀伤细胞。荷瘤动物脾细胞的培养上清液中也含有因子，该因子可诱导正常腹腔渗出细胞在体外对T1699细胞介导的细胞毒性；并且脾细胞用抗θ血清预处理可抑制该因子的产生，但抗小鼠IgG或抗小鼠全γ球蛋白血清和补体则不能抑制。
