Roux F, Guillam C, Bescol-Liversac J
Ann Anat Pathol (Paris). 1978;23(3):173-200.
Principle of the method. Description of the techniques used to obtain the cultures, light and electron microscopic study, biochemical determination of proteins, of DNA, of the incorporation of H3-thymidine, of total and lysosomal acid phosphatase activity. Detailed description of hepatocytes developed in unicellular layer: shape and distribution of the cells, junctions, cellular apparatuses and organelles, cytoplasmic inclusions. Comparison of the hepatocyte in vitro and in vivo. The most obvious differences are related to experimental conditions of geometry and nutrition. Particular note should be taken of the microvillous appendices of the free surface and the underlying explasmic layer. Specific physiological activities are evident, including glycogenogenesis. Discussion of multiplication, growth and cellular differentiation phenomena. Vitality tests. In culture, there may develop a tendency to hypertrophy of the mitochondria, of the dictyosomes, to hyperplasia of the granular or smooth endoplasmic reticulum and above all to increased autophagia.
方法原理。用于获取培养物的技术描述、光镜和电镜研究、蛋白质、DNA、H3-胸苷掺入、总酸性磷酸酶和溶酶体酸性磷酸酶活性的生化测定。对单细胞层中发育的肝细胞的详细描述：细胞的形状和分布、连接、细胞器和细胞器、细胞质内含物。体外和体内肝细胞的比较。最明显的差异与几何形状和营养的实验条件有关。应特别注意自由表面的微绒毛附属物和下面的外质层。明显的特定生理活动，包括糖原生成。对增殖、生长和细胞分化现象的讨论。活力测试。在培养中，可能会出现线粒体、高尔基体肥大，颗粒状或光滑内质网增生，尤其是自噬增加的趋势。
