Hinton J F, Newkirk D K, Fletcher T G, Shungu D C
Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville 72701.
J Magn Reson B. 1994 Sep;105(1):11-6. doi: 10.1006/jmrb.1994.1093.
The magnetization-inversion-transfer NMR technique has been used to determine the activation enthalpy (delta H not equal to) for the transport of the 7Li+, 23Na+, and 39K+ ions through the gramicidin channel incorporated into vesicle membranes. The activation enthalpy obtained for the overall transport of the monovalent cations was found to be in agreement with the selectivity order and single-channel ion conductance values. Furthermore, the magnetization-inversion-transfer technique was also found to be applicable to the channel formed from a synthetic peptide having the same amino acid sequence as the M2 delta transmembrane segment of the nicotinic acetylcholine receptor.
磁化反转转移核磁共振技术已被用于测定7Li+、23Na+和39K+离子通过掺入囊泡膜的短杆菌肽通道的传输活化焓（ΔH≠）。发现单价阳离子整体传输获得的活化焓与选择性顺序和单通道离子电导值一致。此外，还发现磁化反转转移技术适用于由与烟碱型乙酰胆碱受体的M2δ跨膜片段具有相同氨基酸序列的合成肽形成的通道。
