Nakamura Y, Noma M, Kidokoro M, Kobayashi N, Takei M, Kurashima S, Mukaiyama T, Kato S
Blood Transfusion Center, Tokai University Hospital, Kanagawa, Japan.
J Hematother. 1994 Summer;3(2):128-33.
An immunomagnetic separation system has been used to collect CD34+ cells in mobilized blood after treatment with a nylon-wool column. Cell purities were increased from 2.6% preseparation to 94.6% postseparation, with a mean yield 45.2% (n = 4). Forty percent of CD34+ cells separated by the immunomagnetic procedure formed colonies in the presence of hematopoietic growth factors in a limiting dilution assay. Eleven percent of these clones proliferated to over 10(5) cells and contained megakaryocytes.
一种免疫磁珠分离系统已被用于在尼龙棉柱处理后收集动员血液中的CD34+细胞。细胞纯度从分离前的2.6%提高到分离后的94.6%，平均回收率为45.2%（n = 4）。在有限稀释分析中，通过免疫磁珠程序分离的CD34+细胞中有40%在造血生长因子存在的情况下形成集落。这些克隆中有11%增殖到超过10(5)个细胞，并含有巨核细胞。
