Oude Weernink P A, Ottenhoff-Kalff A E, Vendrig M P, van Beurden E A, Staal G E, Rijksen G
Department of Hematology, Laboratory of Medical Enzymology, University Hospital Utrecht, The Netherlands.
FEBS Lett. 1994 Oct 3;352(3):296-300. doi: 10.1016/0014-5793(94)00977-5.
To study the relationship between the tyrosine kinase c-Src and the epidermal growth factor receptor (EGF-R), we used the breast cancer cell line ZR75-1, which was transfected with the EGF-R. The EGF-R transfected cell line expressed 60 times more EGF-R than a control cell line transfected with the empty vector. In the presence of EGF, the EGF-R over-expressing cell line grew much faster than the control cell line. Both cell lines expressed approximately equal amounts of c-Src. However, the cell line over-expressing the EGF-R showed a twofold enhancement of c-Src kinase activity after EGF stimulation. The activation of c-Src kinase by EGF was confirmed in other EGF-R expressing cell types.
