Meyer J, Hinder F, Stothert J, Traber L D, Herndon D N, Flynn J T, Traber D L
Department of Anesthesiology, University of Texas Medical Branch, Galveston 77555-1091.
J Appl Physiol (1985). 1994 Jun;76(6):2785-93. doi: 10.1152/jappl.1994.76.6.2785.
We evaluated regional blood flows in a hyperdynamic sepsis model and the reversal of increased flows by blockade of nitric oxide (NO) synthase. Seven awake sheep were continuously infused with Escherichia coli endotoxin [lipopolysaccharide (LPS), 10 ng.kg-1.min-1] for 48 h. The NO synthase inhibitor N omega-nitro-L-arginine methyl ester (L-NAME, 25 mg/kg) was injected after 24 h. Blood flows to systemic organs were determined with the radioactive microsphere technique. LPS induced elevation of cardiac index by 36% (P < 0.05) and a fall in systemic vascular resistance index by 37% (P < 0.05) at 0 h [time of L-NAME administration, 24 h after infusion of LPS had begun] L-NAME administration normalized cardiac index [6.1 +/- 0.5 at 4 h posttreatment, 6.1 +/- 0.5 l.min-1.m-2 at -24 h (baseline)] and systemic vascular resistance index (1,333 +/- 105 at 4 h posttreatment, 1,280 +/- 163 dyn.s.cm-5.m2 at -24 h) and reduced all regional blood flows to near-baseline levels for the remainder of the study period (24 h). O2 consumption was unaffected by treatment.
我们评估了高动力性脓毒症模型中的局部血流情况，以及通过一氧化氮（NO）合酶阻断对血流增加的逆转作用。七只清醒的绵羊连续48小时输注大肠杆菌内毒素[脂多糖（LPS），10 ng·kg-1·min-1]。24小时后注射NO合酶抑制剂Nω-硝基-L-精氨酸甲酯（L-NAME，25 mg/kg）。用放射性微球技术测定全身各器官的血流。在0小时[L-NAME给药时间，LPS输注开始后24小时]，LPS使心脏指数升高36%（P<0.05），全身血管阻力指数下降37%（P<0.05）。L-NAME给药使心脏指数恢复正常[治疗后4小时为6.1±0.5，-24小时（基线）为6.1±0.5 l·min-1·m-2]和全身血管阻力指数（治疗后4小时为1333±105，-24小时为1280±163 dyn·s·cm-5·m2），并在研究期剩余时间（24小时）内将所有局部血流降低至接近基线水平。氧耗不受治疗影响。
