Peek R, Pruijn G J, van Venrooij W J
Department of Biochemistry, University of Nijmegen, The Netherlands.
J Immunol. 1994 Nov 1;153(9):4321-9.
Ro ribonucleoprotein particles (Ro RNPs) are evolutionarily conserved cytoplasmic complexes of unknown function. They are composed of several proteins and a small, RNA polymerase III-transcribed Ro or Y RNA. Abs directed against the protein moiety of Ro RNPs are often found in sera of patients suffering from certain autoimmune disorders. The association of one of the Ro proteins, a protein of 52 kDa (Ro52), with Ro RNPs is still questionable. In this study, we have used anti-Ro52 Abs isolated from autoimmune sera to locate the antigenic determinants of Ro52 and to analyze the correlation between regions of Ro52 recognized by these Abs and their ability to immunoprecipitate Ro RNPs. The results indicate that the autoimmune response against Ro52 is heterogeneous and that the exclusive recognition of certain epitopes does not result in immunoprecipitation of Ro RNPs.
Ro核糖核蛋白颗粒（Ro RNPs）是功能未知的进化保守细胞质复合物。它们由几种蛋白质和一种小的、由RNA聚合酶III转录的Ro或Y RNA组成。针对Ro RNPs蛋白质部分的抗体经常在患有某些自身免疫性疾病的患者血清中发现。Ro蛋白之一，一种52 kDa的蛋白质（Ro52）与Ro RNPs的关联仍存在疑问。在本研究中，我们使用从自身免疫血清中分离的抗Ro52抗体来定位Ro52的抗原决定簇，并分析这些抗体识别的Ro52区域与其免疫沉淀Ro RNPs能力之间的相关性。结果表明，针对Ro52的自身免疫反应是异质性的，并且对某些表位的特异性识别不会导致Ro RNPs的免疫沉淀。
