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1
Functional properties and substrate specificity of the cloned L-glutamate/L-aspartate transporter GLAST-1 from rat brain expressed in Xenopus oocytes.在非洲爪蟾卵母细胞中表达的来自大鼠脑的克隆L-谷氨酸/L-天冬氨酸转运体GLAST-1的功能特性和底物特异性
J Neurosci. 1994 Oct;14(10):5759-65. doi: 10.1523/JNEUROSCI.14-10-05759.1994.
2
Electrogenic L-glutamate uptake in Xenopus laevis oocytes expressing a cloned rat brain L-glutamate/L-aspartate transporter (GLAST-1).在表达克隆的大鼠脑L-谷氨酸/L-天冬氨酸转运体（GLAST-1）的非洲爪蟾卵母细胞中的电生性L-谷氨酸摄取。
J Biol Chem. 1993 Jul 15;268(20):14594-6.
3
Comparison of Na+-dependent glutamate transport activity in synaptosomes, C6 glioma, and Xenopus oocytes expressing excitatory amino acid carrier 1 (EAAC1).表达兴奋性氨基酸转运体1（EAAC1）的突触体、C6胶质瘤细胞和非洲爪蟾卵母细胞中钠依赖性谷氨酸转运活性的比较。
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Structure, expression, and functional analysis of a Na(+)-dependent glutamate/aspartate transporter from rat brain.大鼠脑内一种钠离子依赖型谷氨酸/天冬氨酸转运体的结构、表达及功能分析
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Inhibition of the high-affinity brain glutamate transporter GLAST-1 via direct phosphorylation.通过直接磷酸化抑制高亲和力脑谷氨酸转运体GLAST-1
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Localization of N-glycosylation sites and functional role of the carbohydrate units of GLAST-1, a cloned rat brain L-glutamate/L-aspartate transporter.克隆的大鼠脑L-谷氨酸/L-天冬氨酸转运体GLAST-1的N-糖基化位点定位及碳水化合物单元的功能作用
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7
Inhibitory activity of a naturally occurring heterocyclic beta-substituted alanine, beta-(isoxazolin-5-on-4-yl)-L-alanine, on the L-glutamate/L-aspartate transporter (GLAST) expressed in Xenopus oocytes.天然存在的杂环β-取代丙氨酸β-（异恶唑啉-5-酮-4-基）-L-丙氨酸对非洲爪蟾卵母细胞中表达的L-谷氨酸/L-天冬氨酸转运体（GLAST）的抑制活性。
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Endogenous L-glutamate transport in oocytes of Xenopus laevis.非洲爪蟾卵母细胞中的内源性L-谷氨酸转运
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9
Functional analysis of the high affinity, Na(+)-dependent glutamate transporter GLAST-1 by site-directed mutagenesis.通过定点诱变对高亲和力、钠依赖性谷氨酸转运体GLAST-1进行功能分析。
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10
Interaction of L-cysteine with a human excitatory amino acid transporter.L-半胱氨酸与人兴奋性氨基酸转运体的相互作用。
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Astrocytes Maintain Glutamate Homeostasis in the CNS by Controlling the Balance between Glutamate Uptake and Release.星形胶质细胞通过控制谷氨酸摄取和释放之间的平衡来维持中枢神经系统中的谷氨酸稳态。
Cells. 2019 Feb 20;8(2):184. doi: 10.3390/cells8020184.
2
Altered phenotype of the vestibular organ in GLAST-1 null mice.GLAST-1 基因敲除小鼠前庭器官表型改变。
J Assoc Res Otolaryngol. 2012 Jun;13(3):323-33. doi: 10.1007/s10162-011-0311-2. Epub 2012 Feb 14.
3
Passive water and urea permeability of a human Na(+)-glutamate cotransporter expressed in Xenopus oocytes.非洲爪蟾卵母细胞中表达的人钠-谷氨酸共转运体的被动水和尿素通透性
J Physiol. 2002 Aug 1;542(Pt 3):817-28. doi: 10.1113/jphysiol.2002.020586.
4
Asymmetry of glia near central synapses favors presynaptically directed glutamate escape.中枢突触附近神经胶质细胞的不对称性有利于突触前导向的谷氨酸逃逸。
Biophys J. 2002 Jul;83(1):125-34. doi: 10.1016/S0006-3495(02)75154-0.
5
Freshly isolated hippocampal CA1 astrocytes comprise two populations differing in glutamate transporter and AMPA receptor expression.新鲜分离的海马CA1区星形胶质细胞由谷氨酸转运体和AMPA受体表达不同的两个群体组成。
J Neurosci. 2001 Oct 15;21(20):7901-8. doi: 10.1523/JNEUROSCI.21-20-07901.2001.
6
Structural features of the glutamate transporter family.谷氨酸转运体家族的结构特征。
Microbiol Mol Biol Rev. 1999 Jun;63(2):293-307. doi: 10.1128/MMBR.63.2.293-307.1999.
7
Optical detection of synaptically induced glutamate transport in hippocampal slices.海马切片中突触诱导的谷氨酸转运的光学检测。
J Neurosci. 1999 Apr 1;19(7):2580-8. doi: 10.1523/JNEUROSCI.19-07-02580.1999.
8
Glutamate transporter currents in bergmann glial cells follow the time course of extrasynaptic glutamate.伯格曼胶质细胞中的谷氨酸转运体电流遵循突触外谷氨酸的时间进程。
Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14821-5. doi: 10.1073/pnas.94.26.14821.
9
Membrane topology of the high-affinity L-glutamate transporter (GLAST-1) of the central nervous system.中枢神经系统高亲和力L-谷氨酸转运体（GLAST-1）的膜拓扑结构。
J Cell Biol. 1996 Dec;135(6 Pt 2):1867-77. doi: 10.1083/jcb.135.6.1867.
10
Noise analysis of the glutamate-activated current in photoreceptors.光感受器中谷氨酸激活电流的噪声分析。
Biophys J. 1996 Feb;70(2):733-42. doi: 10.1016/S0006-3495(96)79613-3.

在非洲爪蟾卵母细胞中表达的来自大鼠脑的克隆L-谷氨酸/L-天冬氨酸转运体GLAST-1的功能特性和底物特异性

Functional properties and substrate specificity of the cloned L-glutamate/L-aspartate transporter GLAST-1 from rat brain expressed in Xenopus oocytes.

作者信息

Klöckner U, Storck T, Conradt M, Stoffel W

机构信息

Department of Physiology, Medical Faculty, University of Cologne, Germany.

出版信息

J Neurosci. 1994 Oct;14(10):5759-65. doi: 10.1523/JNEUROSCI.14-10-05759.1994.

DOI:10.1523/JNEUROSCI.14-10-05759.1994
PMID:7523627
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6576997/
Abstract

The rat brain L-glutamate/L-aspartate transporter GLAST-1 is a member of a family of Na(+)-dependent high-affinity L-glutamate transporters proposed to be involved in the termination and modulation of excitatory neurotransmitter signals. Application of electrophysiological and radiotracer techniques on Xenopus oocytes expressing cloned GLAST-1 revealed that the apparent Km value of the transporter for L-glutamate and Na+ ions did not depend on voltage while the maximal transport rate increased with more negative potentials, indicative of a low-field access channel. The apparent Km value of the transporter for L-glutamate depends on the Na+ concentration, suggesting that substrate and ions are transported by GLAST-1 in a simultaneous manner. All of the L-glutamate uptake blockers tested either were substrates or did not affect the current induced by L-glutamate. The changes in the amplitude of the current induced by simultaneous application of two substrates can be interpreted by a competition for one binding site.

摘要

大鼠脑L-谷氨酸/L-天冬氨酸转运体GLAST-1是Na⁺依赖性高亲和力L-谷氨酸转运体家族的成员之一，该家族成员被认为参与兴奋性神经递质信号的终止和调节。运用电生理学和放射性示踪技术对表达克隆的GLAST-1的非洲爪蟾卵母细胞进行研究，结果显示，该转运体对L-谷氨酸和Na⁺离子的表观Km值不依赖于电压，而最大转运速率随电位越负而增加，这表明存在一个低场通透通道。该转运体对L-谷氨酸的表观Km值取决于Na⁺浓度，这表明底物和离子由GLAST-1同时转运。所测试的所有L-谷氨酸摄取阻滞剂要么是底物，要么不影响L-谷氨酸诱导的电流。同时应用两种底物所诱导电流幅度的变化可以通过对一个结合位点的竞争来解释。