Rebel J M, De Boer W I, Thijssen C D, Vermey M, Zwarthoff E C, Van der Kwast T H
Department of Pathology, Erasmus University, Rotterdam, The Netherlands.
J Pathol. 1994 Jul;173(3):283-91. doi: 10.1002/path.1711730312.
Although the cellular turnover of resting urothelium is very low, its regenerative capacity is known to be outstanding. In organotypic mouse urothelial cultures closely mimicking the differentiation and multilayering of normal urothelium, we examined the cell biological mechanisms underlying urothelial regeneration and the specific role of growth factors and several extracellular matrix (ECM) components. Exposure to epidermal growth factor (EGF) and acidic fibroblast growth factor (aFGF) and culture on laminin resulted in enhanced expansion of the urothelium. Microscopy and assessment of proliferative activity revealed that enhanced urothelial expansion due to EGF could be attributed to increased proliferative activity and an increase in cell numbers, whereas aFGF-stimulated expansion must be considered the consequence of increased cellularity and migration. Laminin-enhanced urothelial expansion was shown to be the result of spreading of the entire urothelial organotypic culture. This was associated with a considerable decrease in the number of cell layers. A synergistic effect of growth factors and laminin was not found. This organotypic urothelial cell culture model seems to be very useful in studying strategies to improve urothelial regeneration.
尽管静息尿路上皮的细胞更新率很低，但其再生能力却十分突出。在紧密模拟正常尿路上皮分化和多层化的器官型小鼠尿路上皮培养物中，我们研究了尿路上皮再生的细胞生物学机制以及生长因子和几种细胞外基质（ECM）成分的特定作用。暴露于表皮生长因子（EGF）和酸性成纤维细胞生长因子（aFGF）并在层粘连蛋白上培养，导致尿路上皮的扩增增强。显微镜检查和增殖活性评估表明，EGF引起的尿路上皮扩增增强可归因于增殖活性增加和细胞数量增多，而aFGF刺激的扩增则必须被视为细胞增多和迁移的结果。层粘连蛋白增强的尿路上皮扩增被证明是整个尿路上皮器官型培养物铺展的结果。这与细胞层数的显著减少有关。未发现生长因子和层粘连蛋白的协同作用。这种器官型尿路上皮细胞培养模型似乎在研究改善尿路上皮再生的策略方面非常有用。
